Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   I6G50_RS09605 Genome accession   NZ_CP065691
Coordinates   1913745..1914155 (+) Length   136 a.a.
NCBI ID   WP_232252357.1    Uniprot ID   -
Organism   Lactococcus garvieae strain FDAARGOS_893     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1908745..1919155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G50_RS09570 (I6G50_09565) guaB 1908960..1910441 (+) 1482 WP_003134540.1 IMP dehydrogenase -
  I6G50_RS09575 (I6G50_09570) rpsU 1910567..1910743 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  I6G50_RS09580 (I6G50_09575) comGA 1910854..1911789 (+) 936 WP_197908696.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G50_RS09585 (I6G50_09580) comGB 1911737..1912756 (+) 1020 WP_269668697.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G50_RS09590 (I6G50_09585) comYC 1912779..1913084 (+) 306 WP_197908697.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G50_RS09595 (I6G50_09590) comGD 1913059..1913475 (+) 417 WP_042753018.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6G50_RS09600 (I6G50_09595) comGE 1913507..1913740 (+) 234 WP_003134534.1 competence type IV pilus minor pilin ComGE -
  I6G50_RS09605 (I6G50_09600) comGF 1913745..1914155 (+) 411 WP_232252357.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G50_RS09610 (I6G50_09605) - 1914394..1915239 (+) 846 WP_197908698.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  I6G50_RS09615 (I6G50_09610) - 1915323..1916192 (-) 870 WP_197908699.1 RluA family pseudouridine synthase -
  I6G50_RS09620 (I6G50_09615) - 1916265..1918568 (+) 2304 WP_197908700.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15959.49 Da        Isoelectric Point: 8.0242

>NTDB_id=513029 I6G50_RS09605 WP_232252357.1 1913745..1914155(+) (comGF) [Lactococcus garvieae strain FDAARGOS_893]
MSFTLLEALVALFVLSGSFLLFLGMTKLFHEEIKKSRIDYTQDWQLFCTLMRNELEGARLEKVENNYLYVNKRTPLRFGL
SAQGDFRKTNADGRGYQPLVHDLKKAEIVQNEKQIKIVLVFKQGGEHIFYYQFIEE

Nucleotide


Download         Length: 411 bp        

>NTDB_id=513029 I6G50_RS09605 WP_232252357.1 1913745..1914155(+) (comGF) [Lactococcus garvieae strain FDAARGOS_893]
TTGAGCTTCACACTATTGGAGGCATTAGTTGCTCTATTTGTTTTATCTGGAAGTTTCTTACTCTTTTTAGGAATGACCAA
GCTCTTTCATGAGGAAATAAAAAAATCGAGAATAGATTATACTCAAGATTGGCAATTGTTCTGTACCTTAATGAGAAATG
AACTTGAAGGAGCACGTTTAGAAAAAGTAGAGAATAATTATCTCTATGTAAACAAAAGAACTCCTCTGCGATTTGGGCTT
TCTGCACAAGGAGATTTTCGAAAAACAAATGCAGATGGTCGTGGCTATCAACCCCTGGTACATGATTTAAAAAAGGCAGA
AATTGTTCAGAATGAAAAGCAAATAAAGATAGTCCTCGTTTTTAAGCAAGGAGGTGAGCACATATTTTACTACCAATTTA
TAGAGGAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(33-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

50

98.529

0.493
  comYF Streptococcus mutans UA140

46.324

100

0.463
  comYF Streptococcus mutans UA159

45.588

100

0.456
  comGF/cglF Streptococcus pneumoniae TIGR4

39.706

100

0.397
  comGF/cglF Streptococcus pneumoniae D39

39.706

100

0.397
  comGF/cglF Streptococcus pneumoniae R6

39.706

100

0.397
  comGF/cglF Streptococcus pneumoniae Rx1

39.706

100

0.397
  comGF/cglF Streptococcus mitis NCTC 12261

39.394

97.059

0.382
  comGF/cglF Streptococcus mitis SK321

38.636

97.059

0.375