Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   I6G50_RS09585 Genome accession   NZ_CP065691
Coordinates   1911737..1912756 (+) Length   339 a.a.
NCBI ID   WP_269668697.1    Uniprot ID   -
Organism   Lactococcus garvieae strain FDAARGOS_893     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1906737..1917756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G50_RS09565 (I6G50_09560) - 1907334..1908785 (-) 1452 WP_003134541.1 GH25 family lysozyme -
  I6G50_RS09570 (I6G50_09565) guaB 1908960..1910441 (+) 1482 WP_003134540.1 IMP dehydrogenase -
  I6G50_RS09575 (I6G50_09570) rpsU 1910567..1910743 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  I6G50_RS09580 (I6G50_09575) comGA 1910854..1911789 (+) 936 WP_197908696.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G50_RS09585 (I6G50_09580) comGB 1911737..1912756 (+) 1020 WP_269668697.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G50_RS09590 (I6G50_09585) comYC 1912779..1913084 (+) 306 WP_197908697.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G50_RS09595 (I6G50_09590) comGD 1913059..1913475 (+) 417 WP_042753018.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6G50_RS09600 (I6G50_09595) comGE 1913507..1913740 (+) 234 WP_003134534.1 competence type IV pilus minor pilin ComGE -
  I6G50_RS09605 (I6G50_09600) comGF 1913745..1914155 (+) 411 WP_232252357.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G50_RS09610 (I6G50_09605) - 1914394..1915239 (+) 846 WP_197908698.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  I6G50_RS09615 (I6G50_09610) - 1915323..1916192 (-) 870 WP_197908699.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39282.93 Da        Isoelectric Point: 9.6073

>NTDB_id=513026 I6G50_RS09585 WP_269668697.1 1911737..1912756(+) (comGB) [Lactococcus garvieae strain FDAARGOS_893]
MDISQLLQLRTKKLSLTKQIKLIQLMNNLFSSGFHIGETIDFLEHSGLVEIYFVSKMREGLLRGESLSSILDDLKVSKDI
VTQLSLAETHGNIELTLGLIEKKLSRVLEIRKKLFQVSTYPLILLVFLTFIMLGLKNYLLPQLENNSSMPVYIIQNLPYL
FLFSVLGLVLFFASFRFYFKKRDALANAKFMIKIPFLGGFVKLYLTAYFSREWGNLIAQAVDLRQICLIMQEQRSRIFRE
FGLELIQALDSGQKFEEVLRFHPIFTKELALIIEYGELKSKLGKELMIFSEESWLRFFEKIERAMQFIQPMVFLVVALLI
VLIYAAMLLPIYNNIGVAM

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=513026 I6G50_RS09585 WP_269668697.1 1911737..1912756(+) (comGB) [Lactococcus garvieae strain FDAARGOS_893]
ATGGATATATCTCAGCTGCTACAGCTTCGTACGAAAAAATTGAGTCTAACTAAACAGATCAAACTTATTCAGCTGATGAA
TAATCTATTTTCGAGCGGTTTTCATATAGGTGAAACCATTGATTTTCTTGAACATTCGGGTTTGGTGGAAATATATTTTG
TTTCTAAGATGCGCGAGGGTTTACTAAGAGGAGAGAGTCTTTCGAGTATCTTAGATGACCTCAAAGTATCCAAAGATATT
GTGACACAGTTGTCTTTGGCAGAAACACATGGAAATATCGAACTCACATTAGGACTAATAGAAAAAAAGTTATCTCGAGT
TTTAGAAATTAGAAAAAAATTATTCCAAGTTTCAACCTATCCTTTAATTCTATTGGTCTTCTTAACTTTCATCATGTTAG
GTTTAAAAAATTATTTATTACCACAGTTAGAAAACAACTCAAGTATGCCTGTGTATATCATTCAAAATTTACCTTATCTT
TTTTTATTTAGTGTGTTAGGTTTGGTCCTTTTCTTCGCCAGCTTTCGATTTTATTTTAAGAAGAGAGATGCTCTGGCAAA
TGCAAAATTTATGATAAAAATCCCTTTTTTAGGAGGTTTCGTTAAACTTTACTTGACCGCTTATTTCTCGAGAGAGTGGG
GAAATTTAATTGCCCAAGCAGTAGATTTACGCCAAATTTGTCTCATTATGCAGGAACAAAGGAGTCGCATTTTTAGAGAA
TTTGGTTTAGAGCTGATTCAGGCTTTAGATAGTGGTCAGAAGTTTGAAGAAGTGCTTCGTTTTCATCCGATATTTACAAA
AGAGCTTGCACTAATCATTGAATATGGAGAACTAAAGTCAAAGCTTGGAAAAGAATTAATGATTTTTTCTGAAGAAAGTT
GGTTAAGGTTTTTCGAAAAAATTGAAAGAGCGATGCAGTTTATCCAGCCTATGGTTTTTTTAGTTGTTGCTTTGCTTATT
GTTTTAATATACGCTGCAATGCTACTCCCAATATATAACAATATAGGTGTAGCCATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.869

99.115

0.534
  comGB/cglB Streptococcus mitis NCTC 12261

51.32

100

0.516
  comGB/cglB Streptococcus mitis SK321

51.335

99.41

0.51
  comGB/cglB Streptococcus pneumoniae Rx1

50.742

99.41

0.504
  comGB/cglB Streptococcus pneumoniae D39

50.742

99.41

0.504
  comGB/cglB Streptococcus pneumoniae R6

50.742

99.41

0.504
  comGB/cglB Streptococcus pneumoniae TIGR4

50.742

99.41

0.504
  comYB Streptococcus gordonii str. Challis substr. CH1

50.296

99.705

0.501
  comYB Streptococcus mutans UA140

50.943

93.805

0.478
  comYB Streptococcus mutans UA159

50.943

93.805

0.478