Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KUU73_RS23885 Genome accession   NZ_CP077981
Coordinates   5189531..5189980 (-) Length   149 a.a.
NCBI ID   WP_021264133.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZPPH14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5184531..5194980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUU73_RS23865 (KUU73_23860) ampD 5185223..5185789 (-) 567 WP_009875882.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KUU73_RS23870 (KUU73_23865) - 5185940..5188195 (-) 2256 WP_009875881.1 DUF1631 domain-containing protein -
  KUU73_RS23875 (KUU73_23870) nadC 5188481..5189329 (+) 849 WP_016264270.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KUU73_RS23885 (KUU73_23880) pilA 5189531..5189980 (-) 450 WP_021264133.1 pilin Machinery gene
  KUU73_RS23890 (KUU73_23885) pilB 5190212..5191912 (+) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KUU73_RS23895 (KUU73_23890) pilC 5191916..5193136 (+) 1221 WP_031634696.1 type 4a pilus biogenesis protein PilC Machinery gene
  KUU73_RS23900 (KUU73_23895) pilD 5193140..5194012 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  KUU73_RS23905 (KUU73_23900) coaE 5194009..5194620 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  KUU73_RS23910 (KUU73_23905) yacG 5194617..5194817 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15537.82 Da        Isoelectric Point: 7.7818

>NTDB_id=512380 KUU73_RS23885 WP_021264133.1 5189531..5189980(-) (pilA) [Pseudomonas aeruginosa strain ZPPH14]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGTNIKIGTDPSTATETYV
GVAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=512380 KUU73_RS23885 WP_021264133.1 5189531..5189980(-) (pilA) [Pseudomonas aeruginosa strain ZPPH14]
ATGAAAGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG
AAGAGTCTCTGTCGCGTGGAATTGCTGGTACCAATATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT
GGGGTTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.667

100

0.691
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.358

100

0.47
  pilA Acinetobacter baumannii strain A118

47.297

99.329

0.47
  comP Acinetobacter baylyi ADP1

43.59

100

0.456
  pilA Vibrio cholerae C6706

42.949

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.949

100

0.45
  pilA Vibrio cholerae strain A1552

42.949

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

45.578

98.658

0.45
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.455

95.973

0.436
  pilA Acinetobacter nosocomialis M2

41.429

93.96

0.389
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

38.514

99.329

0.383