Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IV454_RS04435 Genome accession   NZ_CP065053
Coordinates   977209..977643 (-) Length   144 a.a.
NCBI ID   WP_282961403.1    Uniprot ID   -
Organism   Massilia antarctica strain P8398     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972209..982643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IV454_RS04415 (IV454_04410) - 972622..973068 (-) 447 WP_282961402.1 pilin -
  IV454_RS04420 (IV454_04415) pilA/pilAI 973355..973789 (-) 435 WP_282961401.1 pilin Machinery gene
  IV454_RS04425 (IV454_04420) - 974158..975625 (-) 1468 Protein_864 IS66 family transposase -
  IV454_RS04430 (IV454_04425) - 975722..977010 (-) 1289 Protein_865 IS66 family transposase -
  IV454_RS04435 (IV454_04430) pilA/pilAI 977209..977643 (-) 435 WP_282961403.1 pilin Machinery gene
  IV454_RS33295 (IV454_04435) - 978180..978623 (-) 444 WP_206090486.1 pilin -
  IV454_RS04445 (IV454_04440) - 978839..979432 (-) 594 WP_206090487.1 FHA domain-containing protein -
  IV454_RS04450 (IV454_04445) sucD 979521..980402 (-) 882 WP_099874964.1 succinate--CoA ligase subunit alpha -
  IV454_RS04455 (IV454_04450) sucC 980428..981600 (-) 1173 WP_054268264.1 ADP-forming succinate--CoA ligase subunit beta -
  IV454_RS04460 (IV454_04455) - 981675..982241 (-) 567 WP_206090488.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15031.77 Da        Isoelectric Point: 8.4917

>NTDB_id=506257 IV454_RS04435 WP_282961403.1 977209..977643(-) (pilA/pilAI) [Massilia antarctica strain P8398]
MKSMPMMKKAQAGFTLIELMIVVAIIGILAAVALPAYQDYVAKSQVAAALAEITPGKVQVETKMAEGTAVALATDVGLQL
STKRCTKIDAIVATTGAGSIECTIQGNADVTGATIKWERLATGVWTCSSSLVAPKDKFVPKECK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=506257 IV454_RS04435 WP_282961403.1 977209..977643(-) (pilA/pilAI) [Massilia antarctica strain P8398]
ATGAAATCGATGCCAATGATGAAAAAAGCGCAAGCTGGTTTTACCCTGATCGAACTGATGATCGTCGTTGCAATTATCGG
TATCTTGGCTGCGGTGGCACTGCCGGCATACCAAGATTATGTTGCGAAATCGCAAGTTGCTGCTGCCCTGGCTGAAATTA
CTCCGGGTAAAGTGCAAGTAGAAACCAAAATGGCTGAAGGCACCGCTGTTGCTCTTGCTACCGACGTCGGCCTCCAATTG
TCGACCAAGCGTTGCACCAAGATCGACGCAATCGTTGCTACGACCGGCGCAGGTAGCATCGAGTGCACGATCCAAGGCAA
TGCAGACGTCACGGGCGCAACCATTAAGTGGGAGCGTCTTGCTACCGGCGTCTGGACATGTAGCTCGAGCCTGGTCGCTC
CGAAAGACAAATTCGTACCAAAAGAATGCAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.694

100

0.507
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.205

100

0.458
  pilA Acinetobacter baumannii strain A118

45.652

95.833

0.437
  pilA Pseudomonas aeruginosa PAK

40.789

100

0.431
  pilA Ralstonia pseudosolanacearum GMI1000

34.104

100

0.41
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.726

100

0.403
  pilA Vibrio cholerae strain A1552

39.726

100

0.403
  pilA Vibrio cholerae C6706

39.726

100

0.403
  pilA2 Legionella pneumophila str. Paris

37.584

100

0.389
  pilA2 Legionella pneumophila strain ERS1305867

36.667

100

0.382
  comP Acinetobacter baylyi ADP1

36.242

100

0.375