Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IV454_RS04420 Genome accession   NZ_CP065053
Coordinates   973355..973789 (-) Length   144 a.a.
NCBI ID   WP_282961401.1    Uniprot ID   -
Organism   Massilia antarctica strain P8398     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 968031..975015 973355..973789 within 0


Gene organization within MGE regions


Location: 968031..975015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IV454_RS04385 (IV454_04380) - 969583..970029 (-) 447 WP_282961400.1 pilin -
  IV454_RS04390 (IV454_04385) pilA/pilAI 970316..970750 (-) 435 WP_282961401.1 pilin Machinery gene
  IV454_RS04395 (IV454_04390) - 971122..971550 (-) 429 Protein_858 transposase -
  IV454_RS04400 (IV454_04395) - 971647..971841 (+) 195 WP_229522062.1 transposase -
  IV454_RS04405 (IV454_04400) - 971838..972008 (+) 171 WP_206090484.1 hypothetical protein -
  IV454_RS04410 (IV454_04405) tnpB 972005..972316 (+) 312 WP_206090485.1 IS66 family insertion sequence element accessory protein TnpB -
  IV454_RS04415 (IV454_04410) - 972622..973068 (-) 447 WP_282961402.1 pilin -
  IV454_RS04420 (IV454_04415) pilA/pilAI 973355..973789 (-) 435 WP_282961401.1 pilin Machinery gene

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14946.71 Da        Isoelectric Point: 9.2457

>NTDB_id=506256 IV454_RS04420 WP_282961401.1 973355..973789(-) (pilA/pilAI) [Massilia antarctica strain P8398]
MKSMKMMKKAQAGFTLIELMIVVAIIGILAAVALPAYQDYVAKSQVAAGLAEITPGKVQVETKMAEGTAVALATDVGLQL
STKRCSKIDAIVATTGAGSIECTLQGNADVTGAVIKWSRTAAGVWACTSTLVAPKDKFVPKECK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=506256 IV454_RS04420 WP_282961401.1 973355..973789(-) (pilA/pilAI) [Massilia antarctica strain P8398]
ATGAAATCGATGAAAATGATGAAAAAAGCACAAGCCGGTTTTACCTTGATCGAACTGATGATCGTCGTTGCAATTATCGG
TATCTTGGCTGCTGTGGCATTGCCTGCATATCAGGACTATGTTGCTAAATCGCAAGTTGCTGCTGGCTTGGCTGAAATCA
CCCCAGGTAAAGTACAAGTAGAAACCAAAATGGCTGAAGGCACCGCTGTTGCTCTTGCTACCGACGTCGGCCTCCAATTG
TCGACCAAGCGCTGCTCCAAAATCGATGCAATCGTTGCTACGACGGGCGCTGGTTCCATCGAGTGCACGCTCCAAGGCAA
TGCAGACGTCACGGGCGCAGTCATTAAGTGGTCGCGTACTGCTGCCGGCGTTTGGGCGTGCACCTCGACCTTGGTCGCTC
CTAAAGACAAGTTCGTACCAAAAGAATGCAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

52.482

97.917

0.514
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.205

100

0.458
  pilA Acinetobacter baumannii strain A118

47.101

95.833

0.451
  pilA Pseudomonas aeruginosa PAK

42.105

100

0.444
  pilA Vibrio cholerae C6706

39.216

100

0.417
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.216

100

0.417
  pilA Vibrio cholerae strain A1552

39.216

100

0.417
  pilA2 Legionella pneumophila str. Paris

39.597

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

38.667

100

0.403
  pilA Ralstonia pseudosolanacearum GMI1000

33.918

100

0.403
  comP Acinetobacter baylyi ADP1

38.255

100

0.396
  pilA Vibrio parahaemolyticus RIMD 2210633

40.741

93.75

0.382