Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DKB71_RS28785 Genome accession   NZ_CP064949
Coordinates   6333026..6333445 (-) Length   139 a.a.
NCBI ID   WP_274074572.1    Uniprot ID   -
Organism   Pseudomonas sp. PLMAX     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6328026..6338445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKB71_RS28770 (DKB71_28435) - 6328079..6329095 (+) 1017 WP_408598898.1 class I SAM-dependent rRNA methyltransferase -
  DKB71_RS28775 (DKB71_28440) - 6329189..6332143 (+) 2955 WP_408598900.1 DUF748 domain-containing protein -
  DKB71_RS28780 (DKB71_28445) - 6332364..6332717 (-) 354 WP_274074573.1 BON domain-containing protein -
  DKB71_RS28785 (DKB71_28450) pilA 6333026..6333445 (-) 420 WP_274074572.1 pilin Machinery gene
  DKB71_RS28790 (DKB71_28455) - 6333457..6333630 (+) 174 WP_408598903.1 hypothetical protein -
  DKB71_RS28795 (DKB71_28460) pilC 6333730..6334947 (+) 1218 WP_408598905.1 type II secretion system F family protein Machinery gene
  DKB71_RS28800 (DKB71_28465) pilD 6334949..6335821 (+) 873 WP_323627336.1 A24 family peptidase Machinery gene
  DKB71_RS28805 (DKB71_28470) coaE 6335818..6336441 (+) 624 WP_408598907.1 dephospho-CoA kinase -
  DKB71_RS28810 (DKB71_28475) yacG 6336438..6336638 (+) 201 WP_060543361.1 DNA gyrase inhibitor YacG -
  DKB71_RS28815 (DKB71_28480) - 6336847..6337407 (+) 561 WP_274074567.1 magnesium transporter -
  DKB71_RS28820 (DKB71_28485) - 6337440..6337757 (-) 318 WP_408598910.1 cytochrome C oxidase subunit IV family protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14178.23 Da        Isoelectric Point: 6.1855

>NTDB_id=505764 DKB71_RS28785 WP_274074572.1 6333026..6333445(-) (pilA) [Pseudomonas sp. PLMAX]
MNNQKGFTLIELLIVVAIIGILATFAIPAYSKYQARAKVTAGLAEISALKVAFEDVINQGDDPDLSNVGGAALTSNCTTS
ASGKATDGSGTIGCTILNAPAQVLNKTITLSRTVAGWTCATTVDQEYAPKGCTGVAPKA

Nucleotide


Download         Length: 420 bp        

>NTDB_id=505764 DKB71_RS28785 WP_274074572.1 6333026..6333445(-) (pilA) [Pseudomonas sp. PLMAX]
TTGAACAATCAAAAAGGTTTTACCCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCGAT
ACCGGCTTACTCCAAGTACCAGGCGCGGGCCAAGGTGACGGCGGGGCTGGCCGAGATATCGGCGTTGAAGGTGGCGTTCG
AGGATGTGATCAATCAAGGCGACGACCCTGATTTGTCCAATGTCGGCGGCGCTGCATTAACGTCCAACTGCACGACCTCG
GCCAGCGGTAAAGCCACTGATGGTTCCGGGACTATCGGTTGCACGATCCTTAACGCGCCGGCTCAGGTGCTGAATAAAAC
CATCACCCTTTCGCGTACGGTGGCAGGCTGGACCTGTGCCACCACCGTCGACCAGGAGTACGCGCCGAAAGGCTGCACGG
GGGTTGCCCCGAAAGCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.592

100

0.496
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.715

98.561

0.46
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.681

100

0.453
  pilA Vibrio cholerae strain A1552

44.681

100

0.453
  pilA Vibrio cholerae C6706

44.681

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

97.122

0.432
  comP Acinetobacter baylyi ADP1

40.136

100

0.424
  pilA Pseudomonas aeruginosa PAK

39.597

100

0.424
  pilA2 Legionella pneumophila strain ERS1305867

40.845

100

0.417
  pilA2 Legionella pneumophila str. Paris

38.889

100

0.403