Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   IVG45_RS22915 Genome accession   NZ_CP064653
Coordinates   4702083..4702538 (-) Length   151 a.a.
NCBI ID   WP_196435889.1    Uniprot ID   -
Organism   Methylomonas sp. LL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4697083..4707538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IVG45_RS22025 (IVG45_21975) - 4697552..4698844 (-) 1293 WP_230874692.1 ABC transporter ATP-binding protein -
  IVG45_RS22030 (IVG45_21980) - 4698881..4699711 (-) 831 WP_196435887.1 ABC transporter permease -
  IVG45_RS22035 (IVG45_21985) - 4700094..4702001 (-) 1908 WP_196435888.1 hypothetical protein -
  IVG45_RS22915 (IVG45_21990) pilE 4702083..4702538 (-) 456 WP_196435889.1 pilin Machinery gene
  IVG45_RS22045 (IVG45_21995) - 4702829..4703296 (+) 468 WP_196435890.1 tetratricopeptide repeat protein -
  IVG45_RS22050 (IVG45_22000) - 4703459..4703728 (-) 270 WP_230874693.1 CCDC90 family protein -
  IVG45_RS22070 (IVG45_22020) - 4704251..4704787 (-) 537 WP_196435891.1 hypothetical protein -
  IVG45_RS22075 (IVG45_22025) - 4704998..4706341 (+) 1344 WP_196435892.1 TolC family protein -
  IVG45_RS22080 (IVG45_22030) - 4706362..4707477 (+) 1116 WP_196435893.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15842.02 Da        Isoelectric Point: 4.1237

>NTDB_id=504063 IVG45_RS22915 WP_196435889.1 4702083..4702538(-) (pilE) [Methylomonas sp. LL1]
MNTLSMKKVQQGFTLIELMIVVAIIGILAAIALPAYQDYIIRSQVSEGAALSGGVQTTVSEIWSNTGDLTDADSGALGIP
AATDIKGNYVSQVEVTDGLIEATFESSSPQAANAKIDGLVLQLSPTTTEGSVIWKCKSADIDTKYLPSSCR

Nucleotide


Download         Length: 456 bp        

>NTDB_id=504063 IVG45_RS22915 WP_196435889.1 4702083..4702538(-) (pilE) [Methylomonas sp. LL1]
ATGAACACACTATCCATGAAAAAAGTACAACAAGGCTTTACCTTAATCGAACTGATGATCGTTGTGGCGATCATCGGTAT
CCTGGCGGCGATAGCGTTGCCCGCGTATCAAGATTACATTATCCGCTCCCAAGTATCCGAAGGCGCGGCTTTAAGCGGCG
GCGTACAAACAACCGTTTCCGAAATATGGAGCAACACCGGCGACCTTACCGACGCGGACAGTGGCGCCCTCGGCATACCT
GCCGCGACTGACATTAAAGGAAATTATGTTTCTCAGGTAGAAGTAACAGATGGTTTAATTGAAGCTACATTTGAGAGCTC
CAGTCCTCAAGCAGCCAATGCTAAAATCGATGGCCTAGTATTACAGCTTTCCCCCACGACAACCGAGGGCAGCGTCATTT
GGAAATGCAAAAGCGCAGATATTGATACTAAATATTTACCATCCTCTTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-33)

Pilin (bacterial filament) (Pilin)

(42-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

45.509

100

0.503
  pilE Neisseria gonorrhoeae strain FA1090

44.311

100

0.49
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.715

100

0.444
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44

99.338

0.437
  pilA/pilA1 Eikenella corrodens VA1

40.252

100

0.424
  pilA2 Legionella pneumophila strain ERS1305867

44.138

96.026

0.424
  pilA2 Legionella pneumophila str. Paris

44.138

96.026

0.424
  comP Acinetobacter baylyi ADP1

42.384

100

0.424
  pilA Acinetobacter baumannii strain A118

40.94

98.675

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.61

100

0.404
  pilA Pseudomonas aeruginosa PAK

39.474

100

0.397
  pilA Ralstonia pseudosolanacearum GMI1000

33.929

100

0.377