NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	ITG68_RS24855	Genome accession	NZ_CP064399
Coordinates	5334256..5334705 (-)	Length	149 a.a.
NCBI ID	WP_003112842.1	Uniprot ID	P04739
Organism	Pseudomonas aeruginosa strain QZPH41
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5329256..5339705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ITG68_RS24835 (ITG68_24825)	ampD	5329950..5330516 (-)	567	WP_003104911.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ITG68_RS24840 (ITG68_24830)	-	5330667..5332922 (-)	2256	WP_222831295.1	DUF1631 domain-containing protein	-
ITG68_RS24845 (ITG68_24835)	nadC	5333206..5334054 (+)	849	WP_132709891.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ITG68_RS24855 (ITG68_24845)	pilA	5334256..5334705 (-)	450	WP_003112842.1	type 4a pilus biogenesis protein PilA	Machinery gene
ITG68_RS24860 (ITG68_24850)	pilB	5334938..5336638 (+)	1701	WP_042931548.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ITG68_RS24865 (ITG68_24855)	pilC	5336642..5337862 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
ITG68_RS24870 (ITG68_24860)	pilD	5337866..5338738 (+)	873	WP_222831296.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ITG68_RS24875 (ITG68_24865)	coaE	5338735..5339346 (+)	612	WP_127429831.1	dephospho-CoA kinase	-
ITG68_RS24880 (ITG68_24870)	yacG	5339343..5339543 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 149 a.a. Molecular weight: 15511.78 Da Isoelectric Point: 6.2905

                                >NTDB_id=501795 ITG68_RS24855 WP_003112842.1 5334256..5334705(-) (pilA) [Pseudomonas aeruginosa strain QZPH41]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV

GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide

Download Length: 450 bp

                                >NTDB_id=501795 ITG68_RS24855 WP_003112842.1 5334256..5334705(-) (pilA) [Pseudomonas aeruginosa strain QZPH41]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT

TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC

GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGCGGTGCGGGTGATATTACCTTTAC

CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT

GTAAATCTACCCAGGATCCGATGTTCACCCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
PDB	1PAN
PDB	1PAO
PDB	8TUM
PDB	8TUW
PDB	9EWX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	67.785	100	0.678
pilA	Acinetobacter baumannii strain A118	48	100	0.483
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	48.322	100	0.483
pilA	Vibrio cholerae strain A1552	48.322	100	0.483
pilA	Vibrio cholerae C6706	48.322	100	0.483
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.638	100	0.456
pilA	Vibrio parahaemolyticus RIMD 2210633	44.898	98.658	0.443
comP	Acinetobacter baylyi ADP1	41.83	100	0.43
pilA/pilAII	Pseudomonas stutzeri DSM 10701	44.056	95.973	0.423
pilA2	Legionella pneumophila str. Paris	38.776	98.658	0.383
pilA2	Legionella pneumophila strain ERS1305867	38.095	98.658	0.376

Detailed information NTG0501795