Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ITG68_RS24860 Genome accession   NZ_CP064399
Coordinates   5334938..5336638 (+) Length   566 a.a.
NCBI ID   WP_042931548.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain QZPH41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5329938..5341638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG68_RS24835 (ITG68_24825) ampD 5329950..5330516 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ITG68_RS24840 (ITG68_24830) - 5330667..5332922 (-) 2256 WP_222831295.1 DUF1631 domain-containing protein -
  ITG68_RS24845 (ITG68_24835) nadC 5333206..5334054 (+) 849 WP_132709891.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ITG68_RS24855 (ITG68_24845) pilA 5334256..5334705 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  ITG68_RS24860 (ITG68_24850) pilB 5334938..5336638 (+) 1701 WP_042931548.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ITG68_RS24865 (ITG68_24855) pilC 5336642..5337862 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  ITG68_RS24870 (ITG68_24860) pilD 5337866..5338738 (+) 873 WP_222831296.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ITG68_RS24875 (ITG68_24865) coaE 5338735..5339346 (+) 612 WP_127429831.1 dephospho-CoA kinase -
  ITG68_RS24880 (ITG68_24870) yacG 5339343..5339543 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ITG68_RS24885 (ITG68_24875) - 5339580..5339789 (-) 210 WP_003094660.1 hypothetical protein -
  ITG68_RS24890 (ITG68_24880) - 5339895..5340584 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ITG68_RS24895 (ITG68_24885) - 5340581..5341051 (-) 471 WP_019486073.1 hypothetical protein -
  ITG68_RS24900 (ITG68_24890) - 5341048..5341473 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62356.25 Da        Isoelectric Point: 5.5590

>NTDB_id=501796 ITG68_RS24860 WP_042931548.1 5334938..5336638(+) (pilB) [Pseudomonas aeruginosa strain QZPH41]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSTEKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=501796 ITG68_RS24860 WP_042931548.1 5334938..5336638(+) (pilB) [Pseudomonas aeruginosa strain QZPH41]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAGAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTGGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCACGGAAAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCAGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGACGTACCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGTTGATCGGCACCTTCAAGCTGTATTCGCCAGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCTATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.996

100

0.583
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio cholerae strain A1552

50.526

100

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.799

99.47

0.505
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

39.394

99.117

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366