Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ISN33_RS07400 Genome accession   NZ_CP064002
Coordinates   1713827..1714249 (+) Length   140 a.a.
NCBI ID   WP_081320414.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. translucens strain XtKm33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1708827..1719249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN33_RS07380 (ISN33_07370) - 1709219..1710553 (+) 1335 WP_058359046.1 HAMP domain-containing sensor histidine kinase -
  ISN33_RS07385 (ISN33_07375) coaE 1710680..1711300 (-) 621 WP_206710333.1 dephospho-CoA kinase -
  ISN33_RS07390 (ISN33_07380) - 1711314..1712177 (-) 864 WP_003477103.1 A24 family peptidase -
  ISN33_RS07395 (ISN33_07385) pilC 1712184..1713443 (-) 1260 WP_206710334.1 type II secretion system F family protein Machinery gene
  ISN33_RS07400 (ISN33_07390) pilA/pilAI 1713827..1714249 (+) 423 WP_081320414.1 pilin Machinery gene
  ISN33_RS07405 (ISN33_07395) - 1714321..1714731 (+) 411 WP_081320415.1 pilin -
  ISN33_RS07410 (ISN33_07400) pilB 1715073..1716809 (+) 1737 WP_206710335.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISN33_RS07415 (ISN33_07405) - 1716984..1717436 (-) 453 WP_230812364.1 Fic family protein -
  ISN33_RS07420 (ISN33_07410) - 1717433..1717615 (-) 183 WP_068873434.1 YhfG family protein -
  ISN33_RS07425 (ISN33_07415) pilR 1717804..1719192 (-) 1389 WP_058362147.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14266.45 Da        Isoelectric Point: 8.1054

>NTDB_id=498457 ISN33_RS07400 WP_081320414.1 1713827..1714249(+) (pilA/pilAI) [Xanthomonas translucens pv. translucens strain XtKm33]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLSEITPGKTQAEVLLNEGKDIAAPTDIGLNTPTDRCTI
TATGTASAGTASIACALKGNSKVASGTLTLARTNDGKWACSSAGLDTKYIPTGCTATSAK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=498457 ISN33_RS07400 WP_081320414.1 1713827..1714249(+) (pilA/pilAI) [Xanthomonas translucens pv. translucens strain XtKm33]
ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
GCCGATGTATCAGGATTATGTTGCCAAGTCGCAAGCTACGGCTGGTTTGTCTGAGATCACCCCAGGCAAGACTCAGGCTG
AGGTTTTGTTGAACGAAGGTAAGGATATTGCCGCGCCAACTGACATTGGTCTTAATACCCCAACAGATCGTTGCACCATT
ACGGCAACCGGTACTGCTTCTGCTGGCACTGCATCCATTGCGTGCGCCCTTAAGGGTAATTCAAAAGTTGCAAGCGGCAC
TTTGACTCTCGCTCGTACCAATGATGGTAAGTGGGCATGTTCTTCTGCTGGCTTAGATACCAAATATATCCCGACCGGTT
GCACTGCTACTTCTGCCAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.111

96.429

0.493
  pilA Acinetobacter baumannii strain A118

48.201

99.286

0.479
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.324

97.143

0.45
  pilA Vibrio cholerae C6706

43.151

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.151

100

0.45
  pilA Vibrio cholerae strain A1552

43.151

100

0.45
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.436
  pilE Neisseria gonorrhoeae strain FA1090

33.908

100

0.421
  pilA Ralstonia pseudosolanacearum GMI1000

35.802

100

0.414
  pilE Neisseria gonorrhoeae MS11

34.194

100

0.379
  comP Acinetobacter baylyi ADP1

36.301

100

0.379