Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ISN38_RS15170 Genome accession   NZ_CP063993
Coordinates   3565551..3565973 (+) Length   140 a.a.
NCBI ID   WP_081320414.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. undulosa strain XtLr8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3560551..3570973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN38_RS15150 (ISN38_15145) - 3560936..3562270 (+) 1335 WP_058359046.1 HAMP domain-containing sensor histidine kinase -
  ISN38_RS15155 (ISN38_15150) coaE 3562397..3563017 (-) 621 WP_206710333.1 dephospho-CoA kinase -
  ISN38_RS15160 (ISN38_15155) - 3563031..3563894 (-) 864 WP_003477103.1 A24 family peptidase -
  ISN38_RS15165 (ISN38_15160) pilC 3563901..3565160 (-) 1260 WP_206710334.1 type II secretion system F family protein Machinery gene
  ISN38_RS15170 (ISN38_15165) pilA/pilAI 3565551..3565973 (+) 423 WP_081320414.1 pilin Machinery gene
  ISN38_RS15175 (ISN38_15170) - 3566045..3566455 (+) 411 WP_081320415.1 pilin -
  ISN38_RS15180 (ISN38_15175) pilB 3566797..3568533 (+) 1737 WP_206710335.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ISN38_RS15185 (ISN38_15180) - 3568708..3569160 (-) 453 WP_230812364.1 Fic family protein -
  ISN38_RS15190 (ISN38_15185) - 3569157..3569339 (-) 183 WP_068873434.1 YhfG family protein -
  ISN38_RS15195 (ISN38_15190) pilR 3569528..3570916 (-) 1389 WP_058362147.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14266.45 Da        Isoelectric Point: 8.1054

>NTDB_id=498382 ISN38_RS15170 WP_081320414.1 3565551..3565973(+) (pilA/pilAI) [Xanthomonas translucens pv. undulosa strain XtLr8]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLSEITPGKTQAEVLLNEGKDIAAPTDIGLNTPTDRCTI
TATGTASAGTASIACALKGNSKVASGTLTLARTNDGKWACSSAGLDTKYIPTGCTATSAK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=498382 ISN38_RS15170 WP_081320414.1 3565551..3565973(+) (pilA/pilAI) [Xanthomonas translucens pv. undulosa strain XtLr8]
ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
GCCGATGTATCAGGATTATGTTGCCAAGTCGCAAGCTACGGCTGGTTTGTCTGAGATCACCCCAGGCAAGACTCAGGCTG
AGGTTTTGTTGAACGAAGGTAAGGATATTGCCGCGCCAACTGACATTGGTCTTAATACCCCAACAGATCGTTGCACCATT
ACGGCAACCGGTACTGCTTCTGCTGGCACTGCATCCATTGCGTGCGCCCTTAAGGGTAATTCAAAAGTTGCAAGCGGCAC
TTTGACTCTCGCTCGTACCAATGATGGTAAGTGGGCATGTTCTTCTGCTGGCTTAGATACCAAATATATCCCGACCGGTT
GCACTGCTACTTCTGCCAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.111

96.429

0.493
  pilA Acinetobacter baumannii strain A118

48.201

99.286

0.479
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.324

97.143

0.45
  pilA Vibrio cholerae C6706

43.151

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.151

100

0.45
  pilA Vibrio cholerae strain A1552

43.151

100

0.45
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.436
  pilE Neisseria gonorrhoeae strain FA1090

33.908

100

0.421
  pilA Ralstonia pseudosolanacearum GMI1000

35.802

100

0.414
  pilE Neisseria gonorrhoeae MS11

34.194

100

0.379
  comP Acinetobacter baylyi ADP1

36.301

100

0.379