Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SSAL_RS00720 Genome accession   NC_017594
Coordinates   135590..136531 (+) Length   313 a.a.
NCBI ID   WP_002887018.1    Uniprot ID   A0AAW6D6G7
Organism   Streptococcus salivarius 57.I     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 130590..141531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSAL_RS00710 (Ssal_00140) rpoC 131348..134986 (+) 3639 WP_002887020.1 DNA-directed RNA polymerase subunit beta' -
  SSAL_RS00715 (Ssal_00141) - 135149..135511 (+) 363 WP_002887019.1 DUF1033 family protein -
  SSAL_RS00720 (Ssal_00142) comGA/cglA/cilD 135590..136531 (+) 942 WP_002887018.1 competence type IV pilus ATPase ComGA Machinery gene
  SSAL_RS00725 (Ssal_00143) comYB 136413..137513 (+) 1101 WP_118172207.1 competence type IV pilus assembly protein ComGB Machinery gene
  SSAL_RS00730 (Ssal_00144) comYC 137522..137836 (+) 315 WP_037611125.1 competence type IV pilus major pilin ComGC Machinery gene
  SSAL_RS00735 (Ssal_00145) comYD 137796..138224 (+) 429 WP_014632541.1 competence type IV pilus minor pilin ComGD Machinery gene
  SSAL_RS00740 (Ssal_00146) comGE 138256..138486 (+) 231 WP_013991265.1 competence type IV pilus minor pilin ComGE -
  SSAL_RS00745 (Ssal_00147) comYF 138473..138910 (+) 438 WP_014632542.1 competence type IV pilus minor pilin ComGF Machinery gene
  SSAL_RS00750 comGG 138888..139205 (+) 318 WP_045001363.1 competence type IV pilus minor pilin ComGG -
  SSAL_RS00755 (Ssal_00149) comYH 139250..140206 (+) 957 WP_014632544.1 class I SAM-dependent methyltransferase Machinery gene
  SSAL_RS00760 (Ssal_00150) - 140263..141456 (+) 1194 WP_045001366.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35283.31 Da        Isoelectric Point: 5.7041

>NTDB_id=49373 SSAL_RS00720 WP_002887018.1 135590..136531(+) (comGA/cglA/cilD) [Streptococcus salivarius 57.I]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGQRVSLRLSTVGDYRGLESLVIRVLHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFASENFQSHSSTSWNQQLEGLVKQGYLTEGDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=49373 SSAL_RS00720 WP_002887018.1 135590..136531(+) (comGA/cglA/cilD) [Streptococcus salivarius 57.I]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTTGGCCAGGAGAGGAGATTAATTGATGTATATCGACCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTAGGTTCCTGTGACTATGAT
TGTGGTGATGGTCAAAGAGTTTCTCTGCGTTTGTCTACCGTTGGGGATTATCGTGGCTTAGAAAGTTTAGTTATTCGTGT
TCTACATTCCGAACGTCGAGAATTAGTGTACTGGAATCAAGGAATCCAGCCTATTATGGATGCTTTGGATTATCGTGGAT
TATATCTCTTTGCAGGTCCCGTTGGTTCTGGAAAGACTACGCTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTGATTTCGATTGAGGACCCTGTGGAAATTAAACAGGATAATGTCTTGCAACTCCAGGTGAATCAAGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCACTGCGTCACCGCCCGGATGTTTTGATTATTGGAGAGATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACGGGAGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAACGTCTTCTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCCCTTCAAGGGATTGCCTACATGCGTCTGAT
TAAGGGAGGAGGTGTGATTGATTTTGCCAGTGAAAATTTCCAAAGCCATTCGTCAACCAGCTGGAACCAGCAGTTGGAAG
GCTTGGTTAAACAAGGATATCTCACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comYA Streptococcus mutans UA159

65.176

100

0.652
  comYA Streptococcus mutans UA140

65.176

100

0.652
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.897

99.681

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

51.447

99.361

0.511
  comGA Latilactobacillus sakei subsp. sakei 23K

41.697

86.581

0.361

Multiple sequence alignment