Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IFJ81_RS05745 Genome accession   NZ_CP062066
Coordinates   1367914..1368342 (+) Length   142 a.a.
NCBI ID   WP_057671660.1    Uniprot ID   -
Organism   Xanthomonas campestris strain M28     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1362914..1373342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IFJ81_RS05715 (IFJ81_05715) - 1363017..1363508 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  IFJ81_RS05720 (IFJ81_05720) - 1363505..1363795 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  IFJ81_RS05725 (IFJ81_05725) - 1363870..1364271 (-) 402 WP_040940795.1 SymE family type I addiction module toxin -
  IFJ81_RS05730 (IFJ81_05730) coaE 1364803..1365426 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  IFJ81_RS05735 (IFJ81_05735) - 1365440..1366303 (-) 864 WP_057671659.1 A24 family peptidase -
  IFJ81_RS05740 (IFJ81_05740) pilC 1366310..1367572 (-) 1263 WP_057671824.1 type II secretion system F family protein Machinery gene
  IFJ81_RS05745 (IFJ81_05745) pilA 1367914..1368342 (+) 429 WP_057671660.1 pilin Machinery gene
  IFJ81_RS05750 (IFJ81_05750) pilB 1368478..1370208 (+) 1731 WP_057671826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IFJ81_RS05760 (IFJ81_05760) pilR 1370627..1372021 (-) 1395 WP_057671663.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14272.35 Da        Isoelectric Point: 9.1206

>NTDB_id=485991 IFJ81_RS05745 WP_057671660.1 1367914..1368342(+) (pilA) [Xanthomonas campestris strain M28]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTVRGRVSEAMVAASAAKTVVAENAANGSALDSGWTAPTATNNVASVA
VATATGNITVTTTAKAGGGTIIFAPSANGAALASGTVPTDRISWDCKGGTLAAKYRPAECRT

Nucleotide


Download         Length: 429 bp        

>NTDB_id=485991 IFJ81_RS05745 WP_057671660.1 1367914..1368342(+) (pilA) [Xanthomonas campestris strain M28]
ATGAAAAAGCAACAAGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGCAATCCTTGCCGCCATCGCGCT
GCCGGCTTATCAGGACTACACCGTTCGCGGTCGTGTCTCTGAAGCAATGGTTGCTGCCTCTGCTGCCAAGACGGTTGTGG
CCGAGAATGCTGCGAACGGCTCCGCCCTAGACAGTGGTTGGACCGCTCCTACGGCAACTAACAATGTCGCCAGCGTCGCT
GTTGCTACCGCTACGGGCAATATCACTGTTACTACCACTGCCAAGGCCGGTGGCGGCACGATAATCTTTGCTCCCTCGGC
TAACGGTGCTGCACTGGCTTCCGGTACTGTTCCAACCGATCGTATTTCTTGGGATTGCAAGGGCGGCACCTTGGCTGCCA
AGTACCGTCCAGCTGAATGCCGCACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

52.201

100

0.585
  pilA2 Legionella pneumophila str. Paris

54.795

100

0.563
  pilA2 Legionella pneumophila strain ERS1305867

54.795

100

0.563
  comP Acinetobacter baylyi ADP1

47.771

100

0.528
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.247

100

0.514
  pilA/pilA1 Eikenella corrodens VA1

40

100

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.541

100

0.423
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.136

100

0.415
  pilA Acinetobacter baumannii strain A118

40.426

99.296

0.401
  pilA Vibrio cholerae C6706

33.766

100

0.366
  pilA Vibrio cholerae strain A1552

33.766

100

0.366
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.766

100

0.366