Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LLH_RS03425 Genome accession   NC_017492
Coordinates   668440..668886 (+) Length   148 a.a.
NCBI ID   WP_259285573.1    Uniprot ID   -
Organism   Lactococcus cremoris subsp. cremoris A76     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 663440..673886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLH_RS03405 (llh_3505) - 664703..665263 (+) 561 WP_014572201.1 GNAT family N-acetyltransferase -
  LLH_RS03415 (llh_3515) - 665884..666816 (+) 933 WP_014572202.1 ABC transporter ATP-binding protein -
  LLH_RS03420 (llh_3520) - 666813..668174 (+) 1362 WP_014572203.1 ABC transporter permease -
  LLH_RS03425 (llh_3530) comEA 668440..668886 (+) 447 WP_259285573.1 helix-hairpin-helix domain-containing protein Machinery gene
  LLH_RS03430 (llh_3535) comEC 668867..670201 (+) 1335 WP_080561977.1 ComEC/Rec2 family competence protein Machinery gene
  LLH_RS03435 (llh_3540) - 670206..671096 (-) 891 WP_014572207.1 IS982-like element IS982B family transposase -
  LLH_RS03440 (llh_3545) comEC 671289..671765 (+) 477 WP_014572208.1 MBL fold metallo-hydrolase Machinery gene
  LLH_RS03445 (llh_3550) - 671842..672732 (+) 891 WP_014572209.1 IS982-like element ISLll1 family transposase -
  LLH_RS03450 (llh_3555) - 672753..673058 (+) 306 Protein_668 ComEC/Rec2 family competence protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16042.00 Da        Isoelectric Point: 4.6909

>NTDB_id=47684 LLH_RS03425 WP_259285573.1 668440..668886(+) (comEA) [Lactococcus cremoris subsp. cremoris A76]
MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSTISSSVSNQVS
TTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide


Download         Length: 447 bp        

>NTDB_id=47684 LLH_RS03425 WP_259285573.1 668440..668886(+) (comEA) [Lactococcus cremoris subsp. cremoris A76]
ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG
GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTAATTT
ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTACTATTAGCAGCTCTGTCAGTAATCAAGTTTCA
ACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA
AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG
GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(1-56)

Helix-hairpin-helix motif (HHH_3)

(84-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

95.946

100

0.959
  comEA/celA/cilE Streptococcus mitis SK321

51.02

99.324

0.507
  comEA Latilactobacillus sakei subsp. sakei 23K

44.512

100

0.493
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

48.98

99.324

0.486
  comEA/celA/cilE Streptococcus pneumoniae Rx1

48.299

99.324

0.48
  comEA/celA/cilE Streptococcus pneumoniae D39

48.299

99.324

0.48
  comEA/celA/cilE Streptococcus pneumoniae R6

48.299

99.324

0.48
  comEA/celA/cilE Streptococcus mitis NCTC 12261

47.619

99.324

0.473
  comEA Streptococcus thermophilus LMD-9

44.595

100

0.446
  comEA Staphylococcus aureus MW2

43.151

98.649

0.426
  comEA Staphylococcus aureus N315

41.781

98.649

0.412
  comEA Bacillus subtilis subsp. subtilis str. 168

40.816

99.324

0.405

Multiple sequence alignment