Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   H7F36_RS04980 Genome accession   NZ_CP060296
Coordinates   1059964..1060407 (-) Length   147 a.a.
NCBI ID   WP_187053636.1    Uniprot ID   A0A7G8W2J5
Organism   Variovorax sp. PAMC28562     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1054964..1065407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F36_RS04965 (H7F36_04965) - 1055596..1056612 (-) 1017 WP_187053634.1 glycosyltransferase -
  H7F36_RS04970 (H7F36_04970) - 1056602..1057849 (-) 1248 WP_222620435.1 glycosyltransferase family 2 protein -
  H7F36_RS04975 (H7F36_04975) - 1057827..1059908 (-) 2082 WP_187053635.1 hypothetical protein -
  H7F36_RS04980 (H7F36_04980) pilA2 1059964..1060407 (-) 444 WP_187053636.1 pilin Machinery gene
  H7F36_RS04985 (H7F36_04985) - 1060615..1061421 (-) 807 WP_187053637.1 MBL fold metallo-hydrolase -
  H7F36_RS04990 (H7F36_04990) - 1061449..1063716 (-) 2268 WP_261802503.1 CHASE2 domain-containing protein -
  H7F36_RS04995 (H7F36_04995) - 1063735..1064391 (-) 657 WP_187053638.1 FHA domain-containing protein -
  H7F36_RS05000 (H7F36_05000) - 1064434..1065243 (-) 810 WP_187053639.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15030.38 Da        Isoelectric Point: 9.8883

>NTDB_id=475535 H7F36_RS04980 WP_187053636.1 1059964..1060407(-) (pilA2) [Variovorax sp. PAMC28562]
MNRRNIARRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTTRSKMTEVLLMGAPAKLAVAETTSSIGSLASVTAANSGY
SFPGATKYVSGVAITDATGVVTITSTVPNATGTVTLTPATVGTNTGQLKWTCASGIAGKFLPSECRS

Nucleotide


Download         Length: 444 bp        

>NTDB_id=475535 H7F36_RS04980 WP_187053636.1 1059964..1060407(-) (pilA2) [Variovorax sp. PAMC28562]
ATGAACCGTCGCAATATTGCTCGCCGCGCACAAGCAGGCTTCACCCTTATTGAATTGATGATCGTCGTGGCCATCATTGG
TATCTTGGCTGCTGTGGCTTTGCCGGCTTATCAGGATTACACAACCCGGTCAAAAATGACGGAAGTCCTGTTAATGGGTG
CACCCGCGAAACTTGCTGTCGCTGAGACGACTTCGTCGATAGGCTCGCTGGCTTCAGTCACCGCGGCTAATAGCGGTTAC
AGCTTCCCCGGCGCGACCAAGTATGTTTCAGGTGTCGCGATCACCGACGCGACCGGCGTTGTCACGATTACCTCCACTGT
TCCTAACGCCACCGGTACCGTCACGTTGACCCCTGCCACGGTGGGCACCAACACTGGTCAACTGAAGTGGACCTGCGCAT
CTGGTATTGCTGGGAAGTTCCTGCCTTCGGAATGCCGCAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8W2J5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.555

93.197

0.49
  pilA2 Legionella pneumophila strain ERS1305867

52.555

93.197

0.49
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.838

100

0.476
  pilA Ralstonia pseudosolanacearum GMI1000

39.645

100

0.456
  pilA/pilA1 Eikenella corrodens VA1

42.105

100

0.435
  pilE Neisseria gonorrhoeae MS11

38.75

100

0.422
  pilE Neisseria gonorrhoeae strain FA1090

38.854

100

0.415
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.845

96.599

0.395
  comP Acinetobacter baylyi ADP1

39.189

100

0.395
  pilA Acinetobacter baumannii strain A118

39.189

100

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.912

92.517

0.388
  pilA Pseudomonas aeruginosa PAK

36.601

100

0.381