Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   H4J00_RS05445 Genome accession   NZ_CP059710
Coordinates   1178880..1179305 (+) Length   141 a.a.
NCBI ID   WP_047340102.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzicola strain ML5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1173880..1184305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4J00_RS21695 - 1173925..1174220 (-) 296 Protein_1028 SymE family type I addiction module toxin -
  H4J00_RS05420 (H4J00_05415) - 1174354..1175397 (-) 1044 Protein_1029 IS3 family transposase -
  H4J00_RS05425 (H4J00_05420) - 1175527..1175691 (+) 165 Protein_1030 IS701 family transposase -
  H4J00_RS05430 (H4J00_05425) coaE 1175775..1176383 (-) 609 WP_014504326.1 dephospho-CoA kinase -
  H4J00_RS05435 (H4J00_05430) - 1176397..1177260 (-) 864 WP_047340103.1 A24 family peptidase -
  H4J00_RS05440 (H4J00_05435) pilC 1177267..1178526 (-) 1260 WP_047340536.1 type II secretion system F family protein Machinery gene
  H4J00_RS05445 (H4J00_05440) pilA2 1178880..1179305 (+) 426 WP_047340102.1 pilin Machinery gene
  H4J00_RS05450 (H4J00_05445) - 1179441..1181030 (+) 1590 WP_014504322.1 phosphoethanolamine transferase -
  H4J00_RS05455 (H4J00_05450) - 1181041..1181670 (+) 630 WP_024711608.1 hypothetical protein -
  H4J00_RS05460 (H4J00_05455) - 1181640..1183166 (+) 1527 WP_047340101.1 membrane protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14401.63 Da        Isoelectric Point: 9.0090

>NTDB_id=471517 H4J00_RS05445 WP_047340102.1 1178880..1179305(+) (pilA2) [Xanthomonas oryzae pv. oryzicola strain ML5]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVAETSSSLGGVANVTLANSGYAFPGQTK
YVSAVTIASGTGKVTVTSTVPNATGDIVLTPTDVGGGQLKWTCSSAIATKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=471517 H4J00_RS05445 WP_047340102.1 1178880..1179305(+) (pilA2) [Xanthomonas oryzae pv. oryzicola strain ML5]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTTATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCACCTGCCAAGCTTGCGGTAG
CGGAGACTTCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACGCATTCCCGGGTCAGACGAAG
TATGTCAGCGCCGTCACTATCGCCAGTGGCACGGGTAAAGTCACTGTGACCTCCACGGTGCCCAACGCTACCGGTGACAT
CGTGCTGACCCCGACTGACGTCGGTGGTGGCCAGTTGAAGTGGACCTGCTCTTCCGCCATCGCCACCAAGTACCTGCCGG
CGGAGTGCCGTAACTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50

96.454

0.482
  pilA Ralstonia pseudosolanacearum GMI1000

40.854

100

0.475
  pilA2 Legionella pneumophila strain ERS1305867

49.265

96.454

0.475
  comP Acinetobacter baylyi ADP1

42.857

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

39.241

100

0.44
  pilE Neisseria gonorrhoeae MS11

37.037

100

0.426
  pilA Pseudomonas aeruginosa PAK

36.364

100

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.58

97.872

0.397
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.741

95.745

0.39
  pilA Acinetobacter baumannii strain A118

36.879

100

0.369
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362