Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H3L95_RS06675 Genome accession   NZ_CP059566
Coordinates   1375701..1376828 (-) Length   375 a.a.
NCBI ID   WP_003759034.1    Uniprot ID   C6M6B9
Organism   Neisseria sicca strain DSM 17713     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1370701..1381828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L95_RS06650 (H3L95_06650) dnaK 1371049..1372980 (+) 1932 WP_003759044.1 molecular chaperone DnaK -
  H3L95_RS14210 - 1373115..1373177 (+) 63 Protein_1302 transposase -
  H3L95_RS06660 (H3L95_06660) alr 1373341..1374399 (-) 1059 WP_003759041.1 alanine racemase -
  H3L95_RS06665 (H3L95_06665) gloB 1374674..1375423 (-) 750 WP_003759036.1 hydroxyacylglutathione hydrolase -
  H3L95_RS06675 (H3L95_06675) pilU 1375701..1376828 (-) 1128 WP_003759034.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H3L95_RS06680 (H3L95_06680) - 1377016..1377717 (+) 702 WP_003759032.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  H3L95_RS06685 (H3L95_06685) lepA 1377859..1379652 (+) 1794 WP_036490455.1 translation elongation factor 4 -
  H3L95_RS06690 (H3L95_06690) lepB 1379652..1380671 (+) 1020 WP_003759029.1 signal peptidase I -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42158.40 Da        Isoelectric Point: 6.4185

>NTDB_id=470733 H3L95_RS06675 WP_003759034.1 1375701..1376828(-) (pilU) [Neisseria sicca strain DSM 17713]
MMTNPPSAKDELFTWLRHMNKYKGSDLFITTNFPPAMKVDGKITPITDEPLTSEKCMEIAFSIMSTKQIEEFSSTNECNF
AISLPDTSRFRVNAMVQRGATALVFRSITSKIPNFDSLNLPPVLKDVVMKKRGLVIFVGGTGSGKSTSLAAMVDHRNENC
QDHIITIEDPIEFVHQHKKSIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCLATLHAN
NSNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLILREGGKGRVAAVEILLNSPIISEMIQKGEIHGIKEMMKKSTA
MGMQTFDQALYELYERGDISFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=470733 H3L95_RS06675 WP_003759034.1 1375701..1376828(-) (pilU) [Neisseria sicca strain DSM 17713]
ATGATGACCAATCCTCCCAGCGCAAAAGACGAATTGTTCACTTGGCTGCGCCATATGAACAAATACAAAGGCTCCGACCT
CTTTATCACCACCAATTTTCCGCCCGCGATGAAAGTGGACGGCAAAATCACGCCGATTACCGACGAGCCGCTGACCTCCG
AAAAATGTATGGAAATCGCTTTTTCGATTATGTCCACCAAACAAATCGAAGAGTTTTCCTCCACCAACGAATGCAACTTC
GCCATCAGCCTGCCCGACACCAGCCGCTTCCGTGTCAATGCCATGGTGCAGCGCGGCGCGACGGCGTTGGTGTTCCGCTC
CATTACCAGCAAAATTCCGAATTTTGACAGCCTCAATCTGCCCCCCGTCTTGAAAGACGTGGTGATGAAAAAACGTGGCC
TCGTGATTTTCGTCGGCGGTACCGGCTCGGGCAAATCCACCTCACTCGCCGCGATGGTTGACCACCGCAACGAAAATTGC
CAAGACCACATCATCACCATCGAAGACCCGATTGAGTTCGTCCATCAACACAAAAAAAGCATCATTACCCAGCGCGAAGT
CGGCGTGGATACCGAAAACTGGTTTGCCGCGCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATCCTCATCGGCGAGA
TTCGCGACCGCGAAACCATGGACTACGCCTTGGCGTTCGCCGAAACAGGCCACCTCTGTCTCGCCACGCTGCACGCCAAC
AACTCCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCACCCAGCTTTTGACCGACTTGTCGCT
CAACCTGCAAGCCTTCATCTCGCAACGCCTGATTCTCCGTGAAGGTGGAAAGGGCCGCGTAGCCGCAGTGGAAATCCTGC
TCAATTCGCCGATTATTTCCGAAATGATTCAAAAAGGCGAAATCCACGGCATCAAAGAAATGATGAAAAAATCTACCGCC
ATGGGCATGCAAACCTTCGACCAAGCGCTCTACGAGCTGTACGAACGTGGCGATATCAGCTTCCAAGACGCCATTAAAAA
CGCCGACTCCGCCCACGACCTGCGTTTGGACATCCAACTGCGCAGTCGCCGCGCCCAAAACGCCGGCCCGGACTTGGAAC
TGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6M6B9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.239

93.867

0.547
  pilU Acinetobacter baylyi ADP1

57.726

91.467

0.528
  pilU Vibrio cholerae strain A1552

52.368

95.733

0.501
  pilT Acinetobacter nosocomialis M2

43.519

86.4

0.376
  pilT Acinetobacter baumannii D1279779

43.519

86.4

0.376
  pilT Acinetobacter baumannii strain A118

43.519

86.4

0.376
  pilT Legionella pneumophila strain Lp02

43.21

86.4

0.373
  pilT Legionella pneumophila strain ERS1305867

43.21

86.4

0.373
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.042

88.8

0.373
  pilT Pseudomonas aeruginosa PAK

42.073

87.467

0.368
  pilT Acinetobacter baylyi ADP1

41.768

87.467

0.365
  pilT Neisseria meningitidis 8013

40.299

89.333

0.36