Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HZ322_RS10835 Genome accession   NZ_CP059049
Coordinates   2235674..2236612 (-) Length   312 a.a.
NCBI ID   WP_026138968.1    Uniprot ID   A0AAE4NTI8
Organism   Lactococcus lactis strain N8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2230674..2241612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ322_RS10795 (HZ322_10800) - 2231562..2232404 (-) 843 WP_017865154.1 metal ABC transporter substrate-binding protein -
  HZ322_RS10800 (HZ322_10805) - 2232401..2232838 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  HZ322_RS10805 (HZ322_10810) comGG 2232919..2233203 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  HZ322_RS10810 (HZ322_10815) comGF 2233242..2233688 (-) 447 WP_032948129.1 competence type IV pilus minor pilin ComGF Machinery gene
  HZ322_RS10815 (HZ322_10820) comGE 2233651..2233947 (-) 297 WP_017865157.1 competence type IV pilus minor pilin ComGE Machinery gene
  HZ322_RS10820 (HZ322_10825) comGD 2233919..2234350 (-) 432 WP_026138965.1 competence type IV pilus minor pilin ComGD Machinery gene
  HZ322_RS10825 (HZ322_10830) comGC 2234310..2234645 (-) 336 WP_032948139.1 competence type IV pilus major pilin ComGC Machinery gene
  HZ322_RS10830 (HZ322_10835) comGB 2234707..2235780 (-) 1074 WP_080613278.1 competence type IV pilus assembly protein ComGB Machinery gene
  HZ322_RS10835 (HZ322_10840) comGA 2235674..2236612 (-) 939 WP_026138968.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35709.96 Da        Isoelectric Point: 6.1811

>NTDB_id=466832 HZ322_RS10835 WP_026138968.1 2235674..2236612(-) (comGA) [Lactococcus lactis strain N8]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQMGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=466832 HZ322_RS10835 WP_026138968.1 2235674..2236612(-) (comGA) [Lactococcus lactis strain N8]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAAATGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.09

99.679

0.519
  comYA Streptococcus mutans UA140

51.923

100

0.519
  comYA Streptococcus mutans UA159

51.923

100

0.519
  comYA Streptococcus gordonii str. Challis substr. CH1

51.438

100

0.516