Detailed information NTG0466831

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: HZ322_RS10830
• Genome accession: NZ_CP059049
• Coordinates: 2234707..2235780 (-)
• Length: 357 a.a.
• NCBI ID: WP_080613278.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain N8
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2229707..2240780

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZ322_RS10785 (HZ322_10790)	-	2229846..2230655 (-)	810	WP_014570791.1	metal ABC transporter permease	-
HZ322_RS10790 (HZ322_10795)	-	2230648..2231385 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
HZ322_RS10795 (HZ322_10800)	-	2231562..2232404 (-)	843	WP_017865154.1	metal ABC transporter substrate-binding protein	-
HZ322_RS10800 (HZ322_10805)	-	2232401..2232838 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
HZ322_RS10805 (HZ322_10810)	comGG	2232919..2233203 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
HZ322_RS10810 (HZ322_10815)	comGF	2233242..2233688 (-)	447	WP_032948129.1	competence type IV pilus minor pilin ComGF	Machinery gene
HZ322_RS10815 (HZ322_10820)	comGE	2233651..2233947 (-)	297	WP_017865157.1	competence type IV pilus minor pilin ComGE	Machinery gene
HZ322_RS10820 (HZ322_10825)	comGD	2233919..2234350 (-)	432	WP_026138965.1	competence type IV pilus minor pilin ComGD	Machinery gene
HZ322_RS10825 (HZ322_10830)	comGC	2234310..2234645 (-)	336	WP_032948139.1	competence type IV pilus major pilin ComGC	Machinery gene
HZ322_RS10830 (HZ322_10835)	comGB	2234707..2235780 (-)	1074	WP_080613278.1	competence type IV pilus assembly protein ComGB	Machinery gene
HZ322_RS10835 (HZ322_10840)	comGA	2235674..2236612 (-)	939	WP_026138968.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40338.57 Da
• Isoelectric Point: 10.3000

>NTDB_id=466831 HZ322_RS10830 WP_080613278.1 2234707..2235780(-) (comGB) [Lactococcus lactis strain N8]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWVLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLLKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=466831 HZ322_RS10830 WP_080613278.1 2234707..2235780(-) (comGB) [Lactococcus lactis strain N8]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGTCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTAAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTTGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	82.991	95.518	0.793
comYB	Streptococcus gordonii str. Challis substr. CH1	53.372	95.518	0.51
comYB	Streptococcus mutans UA140	52.353	95.238	0.499
comYB	Streptococcus mutans UA159	52.059	95.238	0.496
comGB/cglB	Streptococcus mitis SK321	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae Rx1	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae D39	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae R6	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae TIGR4	50.746	93.838	0.476
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471