Detailed information NTG0466827

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: HZ322_RS10810
• Genome accession: NZ_CP059049
• Coordinates: 2233242..2233688 (-)
• Length: 148 a.a.
• NCBI ID: WP_032948129.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain N8
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2228242..2238688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZ322_RS10775 (HZ322_10780)	-	2228388..2229503 (-)	1116	Protein_2116	acyltransferase family protein	-
HZ322_RS10780 (HZ322_10785)	-	2229514..2229807 (+)	294	WP_226898169.1	pseudouridine synthase	-
HZ322_RS10785 (HZ322_10790)	-	2229846..2230655 (-)	810	WP_014570791.1	metal ABC transporter permease	-
HZ322_RS10790 (HZ322_10795)	-	2230648..2231385 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
HZ322_RS10795 (HZ322_10800)	-	2231562..2232404 (-)	843	WP_017865154.1	metal ABC transporter substrate-binding protein	-
HZ322_RS10800 (HZ322_10805)	-	2232401..2232838 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
HZ322_RS10805 (HZ322_10810)	comGG	2232919..2233203 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
HZ322_RS10810 (HZ322_10815)	comGF	2233242..2233688 (-)	447	WP_032948129.1	competence type IV pilus minor pilin ComGF	Machinery gene
HZ322_RS10815 (HZ322_10820)	comGE	2233651..2233947 (-)	297	WP_017865157.1	competence type IV pilus minor pilin ComGE	Machinery gene
HZ322_RS10820 (HZ322_10825)	comGD	2233919..2234350 (-)	432	WP_026138965.1	competence type IV pilus minor pilin ComGD	Machinery gene
HZ322_RS10825 (HZ322_10830)	comGC	2234310..2234645 (-)	336	WP_032948139.1	competence type IV pilus major pilin ComGC	Machinery gene
HZ322_RS10830 (HZ322_10835)	comGB	2234707..2235780 (-)	1074	WP_080613278.1	competence type IV pilus assembly protein ComGB	Machinery gene
HZ322_RS10835 (HZ322_10840)	comGA	2235674..2236612 (-)	939	WP_026138968.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=466827 HZ322_RS10810 WP_032948129.1 2233242..2233688(-) (comGF) [Lactococcus lactis strain N8]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=466827 HZ322_RS10810 WP_032948129.1 2233242..2233688(-) (comGF) [Lactococcus lactis strain N8]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	42.336	92.568	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378