Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   HZ322_RS10815 Genome accession   NZ_CP059049
Coordinates   2233651..2233947 (-) Length   98 a.a.
NCBI ID   WP_017865157.1    Uniprot ID   -
Organism   Lactococcus lactis strain N8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2228651..2238947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ322_RS10780 (HZ322_10785) - 2229514..2229807 (+) 294 WP_226898169.1 pseudouridine synthase -
  HZ322_RS10785 (HZ322_10790) - 2229846..2230655 (-) 810 WP_014570791.1 metal ABC transporter permease -
  HZ322_RS10790 (HZ322_10795) - 2230648..2231385 (-) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  HZ322_RS10795 (HZ322_10800) - 2231562..2232404 (-) 843 WP_017865154.1 metal ABC transporter substrate-binding protein -
  HZ322_RS10800 (HZ322_10805) - 2232401..2232838 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  HZ322_RS10805 (HZ322_10810) comGG 2232919..2233203 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  HZ322_RS10810 (HZ322_10815) comGF 2233242..2233688 (-) 447 WP_032948129.1 competence type IV pilus minor pilin ComGF Machinery gene
  HZ322_RS10815 (HZ322_10820) comGE 2233651..2233947 (-) 297 WP_017865157.1 competence type IV pilus minor pilin ComGE Machinery gene
  HZ322_RS10820 (HZ322_10825) comGD 2233919..2234350 (-) 432 WP_026138965.1 competence type IV pilus minor pilin ComGD Machinery gene
  HZ322_RS10825 (HZ322_10830) comGC 2234310..2234645 (-) 336 WP_032948139.1 competence type IV pilus major pilin ComGC Machinery gene
  HZ322_RS10830 (HZ322_10835) comGB 2234707..2235780 (-) 1074 WP_080613278.1 competence type IV pilus assembly protein ComGB Machinery gene
  HZ322_RS10835 (HZ322_10840) comGA 2235674..2236612 (-) 939 WP_026138968.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 98 a.a.        Molecular weight: 11082.91 Da        Isoelectric Point: 5.6957

>NTDB_id=466828 HZ322_RS10815 WP_017865157.1 2233651..2233947(-) (comGE) [Lactococcus lactis strain N8]
MENLKRKSVKAYLLLESLISIALLAFLVSFIVSSLVQVRQKDTEENQKIEALNVAQMAIESHLTELSINGSDIKIKENQN
FLIISNHGKEIMQLELQS

Nucleotide


Download         Length: 297 bp        

>NTDB_id=466828 HZ322_RS10815 WP_017865157.1 2233651..2233947(-) (comGE) [Lactococcus lactis strain N8]
GTGGAAAATTTAAAAAGAAAATCAGTTAAGGCATATTTGCTTTTGGAAAGTTTAATTAGTATAGCTCTACTCGCTTTTCT
AGTCAGCTTTATCGTAAGTTCTCTTGTGCAAGTAAGACAAAAAGATACGGAAGAAAATCAAAAGATTGAAGCTTTAAACG
TGGCACAAATGGCGATTGAAAGTCATTTAACAGAATTATCAATCAACGGTTCTGATATAAAGATAAAAGAAAACCAAAAT
TTTCTGATTATTAGTAATCATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Lactococcus lactis subsp. cremoris KW2

67.01

98.98

0.663