Detailed information NTG0454901

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: HUS11_RS26735
• Genome accession: NZ_CP054844
• Coordinates: 5615223..5615675 (-)
• Length: 150 a.a.
• NCBI ID: WP_114064701.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain SE5357
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5610223..5620675

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUS11_RS26700 (HUS11_26710)	-	5610679..5611341 (+)	663	WP_033992423.1	DUF3218 family protein	-
HUS11_RS26705 (HUS11_26715)	fliP	5611624..5612376 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
HUS11_RS26710 (HUS11_26720)	fliQ	5612421..5612690 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
HUS11_RS26715 (HUS11_26725)	fliR	5612690..5613466 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
HUS11_RS26720 (HUS11_26730)	-	5613469..5613729 (+)	261	Protein_5317	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
HUS11_RS26725 (HUS11_26735)	-	5614726..5614968 (+)	243	WP_023082027.1	hypothetical protein	-
HUS11_RS26735 (HUS11_26745)	pilA	5615223..5615675 (-)	453	WP_114064701.1	pilin	Machinery gene
HUS11_RS26740 (HUS11_26750)	pilB	5615907..5617607 (+)	1701	WP_121494215.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HUS11_RS26745 (HUS11_26755)	pilC	5617611..5618831 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
HUS11_RS26750 (HUS11_26760)	pilD	5618835..5619707 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HUS11_RS26755 (HUS11_26765)	coaE	5619704..5620315 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
HUS11_RS26760 (HUS11_26770)	yacG	5620312..5620512 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15471.67 Da
• Isoelectric Point: 7.7827

>NTDB_id=454901 HUS11_RS26735 WP_114064701.1 5615223..5615675(-) (pilA) [Pseudomonas aeruginosa strain SE5357]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTEDTTYV
GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide

• Length: 453 bp

>NTDB_id=454901 HUS11_RS26735 WP_114064701.1 5615223..5615675(-) (pilA) [Pseudomonas aeruginosa strain SE5357]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGAAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	68.456	99.333	0.68
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.333	100	0.473
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.671	100	0.46
pilA	Vibrio cholerae strain A1552	43.671	100	0.46
pilA	Vibrio cholerae C6706	43.671	100	0.46
pilA	Vibrio parahaemolyticus RIMD 2210633	46.259	98	0.453
pilA	Acinetobacter baumannii strain A118	44.295	99.333	0.44
pilA2	Legionella pneumophila strain ERS1305867	42.282	99.333	0.42
comP	Acinetobacter baylyi ADP1	40.909	100	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.333	0.413
pilA2	Legionella pneumophila str. Paris	41.611	99.333	0.413