Detailed information NTG0454902

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HUS11_RS26740
• Genome accession: NZ_CP054844
• Coordinates: 5615907..5617607 (+)
• Length: 566 a.a.
• NCBI ID: WP_121494215.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain SE5357
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5610907..5622607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUS11_RS26705 (HUS11_26715)	fliP	5611624..5612376 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
HUS11_RS26710 (HUS11_26720)	fliQ	5612421..5612690 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
HUS11_RS26715 (HUS11_26725)	fliR	5612690..5613466 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
HUS11_RS26720 (HUS11_26730)	-	5613469..5613729 (+)	261	Protein_5317	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
HUS11_RS26725 (HUS11_26735)	-	5614726..5614968 (+)	243	WP_023082027.1	hypothetical protein	-
HUS11_RS26735 (HUS11_26745)	pilA	5615223..5615675 (-)	453	WP_114064701.1	pilin	Machinery gene
HUS11_RS26740 (HUS11_26750)	pilB	5615907..5617607 (+)	1701	WP_121494215.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HUS11_RS26745 (HUS11_26755)	pilC	5617611..5618831 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
HUS11_RS26750 (HUS11_26760)	pilD	5618835..5619707 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HUS11_RS26755 (HUS11_26765)	coaE	5619704..5620315 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
HUS11_RS26760 (HUS11_26770)	yacG	5620312..5620512 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HUS11_RS26765 (HUS11_26775)	-	5620549..5620758 (-)	210	WP_003094660.1	hypothetical protein	-
HUS11_RS26770 (HUS11_26780)	-	5620864..5621553 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
HUS11_RS26775 (HUS11_26785)	-	5621550..5622020 (-)	471	WP_010793791.1	hypothetical protein	-
HUS11_RS26780 (HUS11_26790)	-	5622017..5622442 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62350.25 Da
• Isoelectric Point: 5.8580

>NTDB_id=454902 HUS11_RS26740 WP_121494215.1 5615907..5617607(+) (pilB) [Pseudomonas aeruginosa strain SE5357]
MNNSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPPLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=454902 HUS11_RS26740 WP_121494215.1 5615907..5617607(+) (pilB) [Pseudomonas aeruginosa strain SE5357]
ATGAACAACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCTTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCTATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGCCCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.343	100	0.58
pilB	Acinetobacter baylyi ADP1	57.016	99.47	0.567
pilB	Legionella pneumophila strain ERS1305867	54.947	100	0.549
pilB	Vibrio parahaemolyticus RIMD 2210633	50.709	99.647	0.505
pilF	Neisseria gonorrhoeae MS11	50.356	99.293	0.5
pilB	Vibrio cholerae strain A1552	49.823	99.647	0.496
pilB	Vibrio campbellii strain DS40M4	49.468	99.647	0.493
pilF	Thermus thermophilus HB27	39.292	99.823	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.228	93.11	0.375
pilB/pilB1	Synechocystis sp. PCC 6803	38.764	94.346	0.366