NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	FOC67_RS02465	Genome accession	NZ_CP054572
Coordinates	511918..512367 (+)	Length	149 a.a.
NCBI ID	WP_031627735.1	Uniprot ID	A0A6N0KHJ6
Organism	Pseudomonas sp. FDAARGOS_761
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 506918..517367

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC67_RS02440 (FOC67_02440)	yacG	507081..507281 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
FOC67_RS02445 (FOC67_02445)	coaE	507278..507889 (-)	612	WP_003094654.1	dephospho-CoA kinase	-
FOC67_RS02450 (FOC67_02450)	pilD	507886..508758 (-)	873	WP_009315897.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
FOC67_RS02455 (FOC67_02455)	pilC	508762..509982 (-)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
FOC67_RS02460 (FOC67_02460)	pilB	509986..511686 (-)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FOC67_RS02465 (FOC67_02465)	pilA	511918..512367 (+)	450	WP_031627735.1	type 4a pilus biogenesis protein PilA	Machinery gene
FOC67_RS02475 (FOC67_02475)	nadC	512579..513427 (-)	849	WP_009315899.1	carboxylating nicotinate-nucleotide diphosphorylase	-
FOC67_RS02480 (FOC67_02480)	-	513713..515968 (+)	2256	WP_009315900.1	DUF1631 domain-containing protein	-
FOC67_RS02485 (FOC67_02485)	ampD	516119..516685 (+)	567	WP_003112844.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Sequence

Protein

Download Length: 149 a.a. Molecular weight: 15458.80 Da Isoelectric Point: 8.4770

                                >NTDB_id=453558 FOC67_RS02465 WP_031627735.1 511918..512367(+) (pilA) [Pseudomonas sp. FDAARGOS_761]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV

GVAPDANKLGVIAVAIEDSGAGDISFTFQTGTSSPKNATKAITLSRTADGVWTCKSTQDEMFIPKGCTK

Nucleotide

Download Length: 450 bp

                                >NTDB_id=453558 FOC67_RS02465 WP_031627735.1 511918..512367(+) (pilA) [Pseudomonas sp. FDAARGOS_761]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC

GGCGTCGCTCCGGATGCCAACAAGTTGGGTGTAATTGCTGTGGCAATCGAGGATAGTGGTGCAGGTGATATTTCCTTTAC

CTTCCAGACTGGTACCTCTAGTCCTAAGAACGCCACCAAGGCTATTACTCTGAGTCGTACTGCGGATGGTGTTTGGACTT

GTAAGTCCACTCAGGATGAGATGTTCATTCCGAAAGGCTGCACTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6N0KHJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	68.874	100	0.698
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	49.333	100	0.497
pilA	Vibrio cholerae strain A1552	49.333	100	0.497
pilA	Vibrio cholerae C6706	49.333	100	0.497
pilA	Acinetobacter baumannii strain A118	48.344	100	0.49
pilA/pilAI	Pseudomonas stutzeri DSM 10701	44.667	100	0.45
comP	Acinetobacter baylyi ADP1	41.83	100	0.43
pilA	Vibrio parahaemolyticus RIMD 2210633	43.243	99.329	0.43
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.973	0.416
pilA2	Legionella pneumophila str. Paris	37.931	97.315	0.369
pilA2	Legionella pneumophila strain ERS1305867	37.931	97.315	0.369

Detailed information NTG0453558