Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HUE57_RS07980 Genome accession   NZ_CP054491
Coordinates   1408615..1409040 (-) Length   141 a.a.
NCBI ID   WP_174672999.1    Uniprot ID   A0A6N0HV50
Organism   Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1393707..1408508 1408615..1409040 flank 107


Gene organization within MGE regions


Location: 1393707..1409040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUE57_RS19600 (HUE57_07905) - 1394131..1394397 (+) 267 WP_174672986.1 WbqC family protein -
  HUE57_RS19605 (HUE57_07910) - 1394420..1394782 (+) 363 Protein_1593 WbqC family protein -
  HUE57_RS07915 (HUE57_07915) - 1395249..1395965 (+) 717 WP_174672988.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  HUE57_RS07920 (HUE57_07920) - 1396765..1397265 (+) 501 WP_174672989.1 sulfotransferase -
  HUE57_RS07925 (HUE57_07925) - 1397994..1398491 (+) 498 WP_174672990.1 methyltransferase domain-containing protein -
  HUE57_RS07930 (HUE57_07930) - 1398561..1399151 (+) 591 WP_174672991.1 oligosaccharide flippase family protein -
  HUE57_RS07935 (HUE57_07935) - 1399136..1399804 (+) 669 WP_174672992.1 lipopolysaccharide biosynthesis protein -
  HUE57_RS07940 (HUE57_07940) - 1400222..1400797 (+) 576 WP_174672993.1 hypothetical protein -
  HUE57_RS07945 (HUE57_07945) - 1401021..1401242 (-) 222 WP_174672994.1 hypothetical protein -
  HUE57_RS07950 (HUE57_07950) - 1402922..1403227 (+) 306 WP_174672995.1 hypothetical protein -
  HUE57_RS07955 (HUE57_07955) - 1403994..1404563 (+) 570 Protein_1602 glycosyltransferase -
  HUE57_RS20230 - 1404629..1404838 (+) 210 WP_420885715.1 glycosyltransferase -
  HUE57_RS07960 (HUE57_07960) - 1405129..1406012 (-) 884 Protein_1604 glycosyltransferase family 2 protein -
  HUE57_RS07965 (HUE57_07965) - 1406354..1406695 (-) 342 WP_174672997.1 hypothetical protein -
  HUE57_RS07970 (HUE57_07970) - 1407683..1407952 (-) 270 WP_174672998.1 hypothetical protein -
  HUE57_RS07975 (HUE57_07975) - 1408087..1408508 (-) 422 Protein_1607 pilin -
  HUE57_RS07980 (HUE57_07980) pilA 1408615..1409040 (-) 426 WP_174672999.1 pilin Machinery gene

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14402.51 Da        Isoelectric Point: 9.5021

>NTDB_id=452873 HUE57_RS07980 WP_174672999.1 1408615..1409040(-) (pilA) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
MKKMQQGFTLIELMIVVAIIGILAAIALPAYQDYTKRSHVTEGLNLAAGAKAGVAEYFSSKGSWGSNNTSVGLPPSGSIT
GNAVNSVSVATSIITITYKTEVESGATVTLSASAASGGSIAWTCAAGNATMKSKWLPANCR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=452873 HUE57_RS07980 WP_174672999.1 1408615..1409040(-) (pilA) [Candidatus Reidiella endopervernicosa isolate Santa_Monica_outfall]
ATGAAGAAAATGCAACAGGGTTTCACACTGATCGAACTTATGATCGTAGTCGCGATCATCGGTATTCTGGCAGCCATCGC
ACTACCCGCTTACCAGGACTACACCAAACGCTCACACGTCACCGAGGGCCTGAACCTCGCTGCCGGTGCCAAGGCTGGTG
TTGCTGAGTACTTCTCAAGTAAGGGTTCATGGGGCTCCAACAACACCTCAGTTGGCCTGCCTCCATCAGGCAGCATTACC
GGTAATGCGGTTAATAGCGTAAGCGTAGCTACCAGCATCATTACCATCACGTATAAGACCGAAGTTGAATCAGGTGCAAC
CGTTACACTTAGCGCATCAGCTGCTTCAGGCGGTTCAATTGCATGGACTTGCGCAGCAGGTAACGCAACCATGAAGAGCA
AGTGGCTCCCAGCCAACTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(37-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0HV50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

43.478

100

0.496
  pilA2 Legionella pneumophila strain ERS1305867

48.551

97.872

0.475
  pilE Neisseria gonorrhoeae MS11

41.104

100

0.475
  pilE Neisseria gonorrhoeae strain FA1090

40.625

100

0.461
  pilA2 Legionella pneumophila str. Paris

47.445

97.163

0.461
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.158

100

0.447
  comP Acinetobacter baylyi ADP1

42.282

100

0.447
  pilA/pilA1 Eikenella corrodens VA1

39.744

100

0.44
  pilA/pilAI Pseudomonas stutzeri DSM 10701

35.948

100

0.39
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

100

0.383
  pilA Acinetobacter baumannii strain A118

36.552

100

0.376
  pilA Pseudomonas aeruginosa PAK

33.987

100

0.369