Detailed information NTG0448951

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ST4145_RS06610
• Genome accession: NZ_CP065492
• Coordinates: 1288750..1291146 (-)
• Length: 798 a.a.
• NCBI ID: WP_014608535.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus strain 4145
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1283750..1296146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST4145_RS06590 (ST4145_06530)	stkP/pknB	1283833..1285704 (-)	1872	WP_024009998.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
ST4145_RS06595 (ST4145_06535)	-	1285704..1286441 (-)	738	WP_002946881.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
ST4145_RS06600 (ST4145_06540)	rsmB	1286485..1287807 (-)	1323	WP_014608533.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ST4145_RS06605 (ST4145_06545)	fmt	1287797..1288732 (-)	936	WP_014608534.1	methionyl-tRNA formyltransferase	-
ST4145_RS06610 (ST4145_06550)	priA	1288750..1291146 (-)	2397	WP_014608535.1	primosomal protein N'	Machinery gene
ST4145_RS06615 (ST4145_06555)	rpoZ	1291288..1291602 (-)	315	WP_002951415.1	DNA-directed RNA polymerase subunit omega	-
ST4145_RS06620 (ST4145_06560)	gmk	1291624..1292253 (-)	630	WP_014608537.1	guanylate kinase	-
ST4145_RS06625 (ST4145_06565)	ftsY	1292486..1293877 (-)	1392	WP_024010000.1	signal recognition particle-docking protein FtsY	-
ST4145_RS06630 (ST4145_06570)	-	1293891..1294706 (-)	816	WP_024010001.1	Cof-type HAD-IIB family hydrolase	-
ST4145_RS06635 (ST4145_06575)	-	1294699..1295493 (-)	795	WP_024010002.1	HAD family hydrolase	-
ST4145_RS06640 (ST4145_06580)	-	1295678..1296055 (+)	378	WP_024010003.1	GntR family transcriptional regulator	-

Sequence

Protein

• Length: 798 a.a.
• Molecular weight: 90714.85 Da
• Isoelectric Point: 6.7337

>NTDB_id=448951 ST4145_RS06610 WP_014608535.1 1288750..1291146(-) (priA) [Streptococcus thermophilus strain 4145]
MVLVAQIIVDVPLMQTDRPYSYLVPESMQGQITIGMRIHVPFGKGNRLLQGFVIGFGEQENVRNLPDLKPIAELLDYEPV
LNQDQLDLADQMRHTVFSYKISILKSMLPGLLNSQYDKVIMATDKLSEEERDLFLQGRDHILFSQLSKEQGQAIPRLVGS
GRVTVDYIARDKQNIKTEKHYSVQRGILETLDISQRAKKRLELRDFLLEKNEPGKLVDLHKLFSRDVVKFFIEAGALAIT
EVEVNRADSYFEKVEKTDFLELNAQQAHAVEVMTGQIGHGGKPFLLEGVTGSGKTEVYLHLIERTLAMGKTAIVLVPEIS
LTPQMTNRFISRFGDLVAIMHSGLSDGEKFDEWRKVRSGQAKVVVGARSAIFAPLDNIGAIIIDEEHEATYKQESNPRYH
ARDVALLRSKSHGAILVLGSATPSIESRARAQKGVYHFFELTERANPSAKIPQVEVVDFKDFVGRQEASDFTPPLLEKIR
ERLARKEQVVLLLNRRGYSSFVMCRDCGYVDDCPNCDISLTLHMDTKTMNCHYCDFQKAIPNVCPNCQSRQIRYYGTGTQ
KAYDQLTELFPEARVIRMDVDTTRKKGAHEKLLETFGSGKADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRSAE
RTFQLLTQVAGRAGRADKEGEVIIQTYNPSHYAIRFAQQQDYEGFYAYEMGIRRQLAYPPYFYTVGITLSHKDEEFVVRK
SYDVMACLREHLSDKVTFLGPTPKPIARTHNLYHYQIIIKYRFEDSLEETLNRILDMTQEPENKDLRLIIDHEPQNFT

Nucleotide

• Length: 2397 bp

>NTDB_id=448951 ST4145_RS06610 WP_014608535.1 1288750..1291146(-) (priA) [Streptococcus thermophilus strain 4145]
ATGGTTTTAGTTGCACAAATAATTGTAGATGTTCCTCTAATGCAGACGGACAGGCCTTATAGTTACCTTGTACCAGAATC
CATGCAGGGGCAAATTACTATCGGTATGAGGATTCATGTGCCTTTTGGTAAAGGAAATCGTCTCTTGCAAGGGTTTGTTA
TTGGCTTTGGAGAGCAAGAAAATGTAAGAAATCTGCCAGACTTGAAGCCAATTGCGGAACTTTTGGATTATGAACCAGTC
CTTAATCAGGATCAGCTGGATTTGGCTGACCAAATGCGACACACGGTTTTTTCCTATAAGATTTCCATCTTGAAGTCTAT
GCTTCCGGGTCTCCTTAATTCACAGTATGACAAAGTTATCATGGCAACTGATAAACTGAGCGAAGAGGAGAGGGACCTTT
TTCTTCAGGGGAGAGACCATATTCTCTTCTCTCAATTGTCGAAAGAACAAGGTCAAGCCATTCCGCGTTTGGTGGGATCT
GGTCGCGTAACGGTTGATTACATTGCTAGGGATAAACAAAATATAAAAACGGAGAAACATTATAGTGTTCAAAGAGGCAT
TTTAGAGACTTTGGATATTTCGCAACGAGCCAAGAAGCGGCTGGAGTTGCGGGACTTCTTGTTAGAAAAGAATGAGCCTG
GAAAGTTAGTAGACCTGCATAAACTTTTTTCTCGAGATGTGGTGAAGTTTTTCATTGAGGCTGGAGCTCTAGCCATCACT
GAAGTTGAGGTCAATCGTGCTGATAGCTACTTTGAAAAGGTAGAGAAGACGGATTTTCTAGAGCTTAATGCTCAGCAAGC
ACATGCTGTTGAAGTAATGACAGGTCAGATTGGTCATGGTGGGAAGCCGTTCTTGTTGGAAGGAGTTACGGGGTCAGGTA
AGACTGAGGTTTACCTGCATCTTATTGAGAGAACCTTGGCTATGGGAAAAACTGCCATTGTCTTGGTTCCAGAAATTTCT
CTGACGCCACAAATGACCAATCGTTTCATCTCCCGTTTTGGTGACTTGGTAGCAATCATGCATTCAGGCTTGTCAGATGG
TGAAAAATTTGACGAGTGGCGTAAGGTTAGGTCTGGTCAAGCAAAGGTCGTTGTAGGAGCACGTTCTGCTATCTTTGCTC
CCTTGGACAATATTGGAGCAATCATTATCGATGAGGAGCACGAGGCGACCTATAAGCAGGAATCCAATCCTCGCTATCAT
GCTAGAGATGTGGCCTTGCTTAGGTCCAAAAGTCATGGCGCTATTCTTGTACTTGGTTCAGCCACGCCTTCTATTGAGTC
GCGAGCGAGGGCTCAGAAAGGGGTCTACCATTTTTTTGAATTGACGGAGAGGGCCAATCCTAGTGCTAAAATTCCGCAGG
TTGAAGTGGTGGACTTTAAGGACTTTGTCGGTAGACAAGAGGCGAGTGATTTTACGCCACCTCTCTTGGAGAAAATCCGA
GAGCGATTGGCACGTAAGGAACAAGTCGTTTTGTTGTTGAATCGTCGTGGGTATTCTTCATTTGTCATGTGTCGTGACTG
TGGCTATGTGGATGACTGTCCGAACTGTGATATTTCACTGACTTTGCATATGGATACCAAGACCATGAATTGTCACTACT
GTGATTTTCAAAAAGCTATTCCTAATGTCTGTCCTAATTGCCAGAGTCGTCAGATTCGCTATTATGGGACTGGGACACAG
AAGGCTTATGATCAATTGACGGAACTTTTTCCAGAAGCCAGAGTGATTCGTATGGATGTTGATACTACACGTAAAAAAGG
CGCTCACGAGAAACTTTTAGAGACTTTTGGGAGTGGCAAGGCTGATATTCTGCTAGGCACCCAAATGATTGCCAAGGGCT
TGGATTTTCCCAATGTTACCTTGGTAGGGGTCTTAAATGCGGATACGTCGCTAAATTTACCAGATTTTCGTTCGGCTGAG
CGCACTTTCCAGCTGTTGACTCAGGTGGCTGGTCGTGCTGGTCGTGCGGACAAGGAAGGGGAGGTTATTATTCAGACCTA
TAACCCTAGTCATTATGCCATTCGTTTTGCTCAGCAACAGGATTATGAGGGATTCTATGCCTATGAGATGGGGATTCGCC
GTCAGTTGGCCTACCCGCCATATTTTTATACGGTAGGGATCACTCTTTCCCACAAGGATGAAGAGTTTGTAGTCCGTAAG
AGCTATGATGTCATGGCTTGTTTAAGAGAACATTTGTCGGACAAGGTGACTTTCTTGGGACCAACACCAAAACCTATTGC
CCGAACACATAACCTTTATCATTATCAGATTATTATCAAATATCGTTTTGAGGATAGCTTGGAAGAGACACTTAATAGGA
TCCTTGATATGACTCAGGAACCTGAAAATAAGGATCTACGTTTGATTATCGATCATGAGCCTCAGAATTTTACCTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (260-424)

• Primosomal protein N C-terminal domain (PriA_C) (700-794)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-109)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (513-538)

• Helicase conserved C-terminal domain (Helicase_C) (567-656)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.95	100	0.484