Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HPY08_RS01305 Genome accession   NZ_CP053808
Coordinates   278900..279484 (+) Length   194 a.a.
NCBI ID   WP_162797022.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273900..284484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY08_RS01285 (HPY08_01285) tnpA 274126..274563 (+) 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
  HPY08_RS01290 (HPY08_01295) tnpA 274748..275185 (+) 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
  HPY08_RS01295 (HPY08_01300) - 275274..277751 (-) 2478 WP_162797021.1 penicillin-binding protein 1A -
  HPY08_RS01300 (HPY08_01305) pilM 277894..278910 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  HPY08_RS01305 (HPY08_01310) pilN 278900..279484 (+) 585 WP_162797022.1 PilN domain-containing protein Machinery gene
  HPY08_RS01310 (HPY08_01315) pilO 279477..280067 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY08_RS01315 (HPY08_01320) pilP 280057..280575 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY08_RS01320 (HPY08_01325) pilQ 280601..282316 (+) 1716 WP_175242463.1 type IV pilus secretin PilQ Machinery gene
  HPY08_RS01325 (HPY08_01330) aroK 282503..283027 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY08_RS01330 (HPY08_01335) aroB 283055..284140 (+) 1086 WP_000439804.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22040.49 Da        Isoelectric Point: 9.7294

>NTDB_id=447638 HPY08_RS01305 WP_162797022.1 278900..279484(+) (pilN) [Vibrio cholerae strain SP7G]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEMTGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=447638 HPY08_RS01305 WP_162797022.1 278900..279484(+) (pilN) [Vibrio cholerae strain SP7G]
ATGTTGCATAAGGTCAATTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATTTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATGACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACATAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

98.969

100

0.99
  pilN Vibrio campbellii strain DS40M4

59.887

91.237

0.546