Detailed information NTG0447637

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HPY08_RS01300
• Genome accession: NZ_CP053808
• Coordinates: 277894..278910 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae strain SP7G
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 272894..283910

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPY08_RS01280 (HPY08_01280)	oxyR	273062..273955 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
HPY08_RS01285 (HPY08_01285)	tnpA	274126..274563 (+)	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY08_RS01290 (HPY08_01295)	tnpA	274748..275185 (+)	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY08_RS01295 (HPY08_01300)	-	275274..277751 (-)	2478	WP_162797021.1	penicillin-binding protein 1A	-
HPY08_RS01300 (HPY08_01305)	pilM	277894..278910 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
HPY08_RS01305 (HPY08_01310)	pilN	278900..279484 (+)	585	WP_162797022.1	PilN domain-containing protein	Machinery gene
HPY08_RS01310 (HPY08_01315)	pilO	279477..280067 (+)	591	WP_000157688.1	type 4a pilus biogenesis protein PilO	Machinery gene
HPY08_RS01315 (HPY08_01320)	pilP	280057..280575 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
HPY08_RS01320 (HPY08_01325)	pilQ	280601..282316 (+)	1716	WP_175242463.1	type IV pilus secretin PilQ	Machinery gene
HPY08_RS01325 (HPY08_01330)	aroK	282503..283027 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=447637 HPY08_RS01300 WP_000520444.1 277894..278910(+) (pilM) [Vibrio cholerae strain SP7G]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=447637 HPY08_RS01300 WP_000520444.1 277894..278910(+) (pilM) [Vibrio cholerae strain SP7G]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTACTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGTTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTACCAACGCTTGTCATTGCCTTGTGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485