Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HPY08_RS01310 Genome accession   NZ_CP053808
Coordinates   279477..280067 (+) Length   196 a.a.
NCBI ID   WP_000157688.1    Uniprot ID   A0A655VUM8
Organism   Vibrio cholerae strain SP7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274477..285067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY08_RS01290 (HPY08_01295) tnpA 274748..275185 (+) 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
  HPY08_RS01295 (HPY08_01300) - 275274..277751 (-) 2478 WP_162797021.1 penicillin-binding protein 1A -
  HPY08_RS01300 (HPY08_01305) pilM 277894..278910 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  HPY08_RS01305 (HPY08_01310) pilN 278900..279484 (+) 585 WP_162797022.1 PilN domain-containing protein Machinery gene
  HPY08_RS01310 (HPY08_01315) pilO 279477..280067 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY08_RS01315 (HPY08_01320) pilP 280057..280575 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY08_RS01320 (HPY08_01325) pilQ 280601..282316 (+) 1716 WP_175242463.1 type IV pilus secretin PilQ Machinery gene
  HPY08_RS01325 (HPY08_01330) aroK 282503..283027 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY08_RS01330 (HPY08_01335) aroB 283055..284140 (+) 1086 WP_000439804.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22891.30 Da        Isoelectric Point: 4.4169

>NTDB_id=447639 HPY08_RS01310 WP_000157688.1 279477..280067(+) (pilO) [Vibrio cholerae strain SP7G]
MASLQELELDEISEWPLLPQLLVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=447639 HPY08_RS01310 WP_000157688.1 279477..280067(+) (pilO) [Vibrio cholerae strain SP7G]
ATGGCTAGTTTGCAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGCTGCTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCCCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A655VUM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

99.49

100

0.995
  pilO Vibrio campbellii strain DS40M4

71.429

96.429

0.689