Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   ST90728_RS08945 Genome accession   NZ_CP065479
Coordinates   1732784..1733077 (-) Length   97 a.a.
NCBI ID   WP_011226625.1    Uniprot ID   Q5M2G2
Organism   Streptococcus thermophilus strain 90728     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1727784..1738077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST90728_RS08910 (ST90728_08890) - 1728187..1728630 (-) 444 WP_002946118.1 hypothetical protein -
  ST90728_RS08915 - 1728704..1729129 (-) 426 WP_096811681.1 CPBP family intramembrane glutamic endopeptidase -
  ST90728_RS08920 (ST90728_08900) - 1729372..1729569 (-) 198 WP_002885716.1 helix-turn-helix transcriptional regulator -
  ST90728_RS08925 (ST90728_08905) - 1729818..1731011 (-) 1194 WP_347114096.1 acetate kinase -
  ST90728_RS08930 (ST90728_08910) comGH 1731067..1732023 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  ST90728_RS08935 (ST90728_08915) comGG 1732068..1732385 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG Machinery gene
  ST90728_RS08940 (ST90728_08920) comGF 1732363..1732800 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST90728_RS08945 (ST90728_08925) comGE 1732784..1733077 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE Machinery gene
  ST90728_RS08950 (ST90728_08930) comGD 1733049..1733477 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST90728_RS08955 (ST90728_08935) comGC 1733437..1733763 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  ST90728_RS08960 (ST90728_08940) comGB 1733760..1734860 (-) 1101 WP_116920398.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST90728_RS08965 (ST90728_08945) comGA/cglA/cilD 1734742..1735683 (-) 942 WP_011226629.1 competence type IV pilus ATPase ComGA Machinery gene
  ST90728_RS08970 (ST90728_08950) - 1735764..1736126 (-) 363 WP_014608746.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10844.74 Da        Isoelectric Point: 10.4561

>NTDB_id=447634 ST90728_RS08945 WP_011226625.1 1732784..1733077(-) (comGE) [Streptococcus thermophilus strain 90728]
MVNIKRRSLRGYILIESLVAMAVLVLVSSLILEQINTNRRLMADNLHQQEVLSVATMAVQTKQDQLTLNGIAVTVKRSQQ
GITVYESGKEIIHVSKQ

Nucleotide


Download         Length: 294 bp        

>NTDB_id=447634 ST90728_RS08945 WP_011226625.1 1732784..1733077(-) (comGE) [Streptococcus thermophilus strain 90728]
GTGGTAAATATAAAAAGACGATCTCTTAGAGGTTATATTCTTATTGAAAGTCTGGTAGCTATGGCTGTTTTAGTACTTGT
CAGCAGTCTGATATTGGAACAAATTAATACCAACCGCAGACTAATGGCTGATAATCTCCATCAACAAGAGGTCTTGAGTG
TGGCAACAATGGCAGTTCAGACCAAGCAAGACCAGTTAACGCTGAATGGCATAGCGGTGACAGTTAAACGTAGCCAGCAA
GGTATCACGGTTTATGAATCGGGAAAGGAGATTATTCATGTATCTAAGCAGTAA

Domains

Predicted by InterProScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M2G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Streptococcus mutans UA140

43.299

100

0.433
  comGE Streptococcus mutans UA159

43.299

100

0.433
  comGE Lactococcus lactis subsp. cremoris KW2

36.082

100

0.361