Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HB761_RS09225 Genome accession   NZ_CP050467
Coordinates   1970947..1972701 (+) Length   584 a.a.
NCBI ID   WP_255940587.1    Uniprot ID   A0AAE9SND3
Organism   Vibrio campbellii strain LJC014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1965947..1977701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB761_RS09205 (HB761_09190) pilM 1968246..1969262 (+) 1017 WP_255933102.1 type IV pilus biogenesis protein PilM Machinery gene
  HB761_RS09210 (HB761_09195) pilN 1969246..1969821 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB761_RS09215 (HB761_09200) pilO 1969814..1970407 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB761_RS09220 (HB761_09205) pilP 1970397..1970912 (+) 516 WP_010643498.1 pilus assembly protein PilP Machinery gene
  HB761_RS09225 (HB761_09210) pilQ 1970947..1972701 (+) 1755 WP_255940587.1 type IV pilus secretin PilQ Machinery gene
  HB761_RS09230 (HB761_09215) aroK 1972890..1973408 (+) 519 WP_010443904.1 shikimate kinase AroK -
  HB761_RS09235 (HB761_09220) aroB 1973440..1974540 (+) 1101 WP_255933103.1 3-dehydroquinate synthase -
  HB761_RS09240 (HB761_09225) - 1974594..1976087 (+) 1494 WP_255933104.1 SPOR domain-containing protein -
  HB761_RS09245 (HB761_09230) - 1976167..1976976 (+) 810 WP_025522125.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  HB761_RS09250 (HB761_09235) - 1976993..1977649 (+) 657 WP_255933106.1 site-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63975.92 Da        Isoelectric Point: 4.5178

>NTDB_id=433059 HB761_RS09225 WP_255940587.1 1970947..1972701(+) (pilQ) [Vibrio campbellii strain LJC014]
MKMRHGLTLTPRKLTGLFSLWFSLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHYYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGEGEGEGDDGGSSVGIDDFLNVNLAATSP
NATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVT
PQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGAL
FRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=433059 HB761_RS09225 WP_255940587.1 1970947..1972701(+) (pilQ) [Vibrio campbellii strain LJC014]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTCTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGACACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACTACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCCTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTCGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAACAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGAGAGGGCAACATCAGTATGCTTTCAGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACCTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCTTGTACGATGGCGGCGGTGAAG
GTGAAGGTGAAGGTGATGACGGTGGCAGCAGTGTAGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCA
AATGCGACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACA
AGAATCGAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCG
AGATCCCTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACG
CCACAGATTACGCCTGACAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCAGGCCAAGTGGTAAAAACAGG
CACTGGTGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTCAATAATGGCGAGACAGTCGTATTGG
GCGGTATTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTT
TTCCGTCGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(424-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.801

100

0.988
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.018

97.603

0.752
  pilQ Vibrio cholerae strain A1552

77.018

97.603

0.752
  pilQ Legionella pneumophila strain ERS1305867

40.998

96.062

0.394
  pilQ Legionella pneumophila strain Lp02

41.379

94.349

0.39
  pilQ Pseudomonas aeruginosa PAK

40.035

97.089

0.389
  pilQ Acinetobacter baumannii D1279779

37.271

100

0.384
  pilQ Acinetobacter baumannii strain A118

37.271

100

0.384
  comQ Acinetobacter baylyi ADP1

36.878

99.829

0.368