Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HB761_RS09215 Genome accession   NZ_CP050467
Coordinates   1969814..1970407 (+) Length   197 a.a.
NCBI ID   WP_005432718.1    Uniprot ID   A0A812GEL0
Organism   Vibrio campbellii strain LJC014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1964814..1975407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB761_RS09200 (HB761_09185) - 1965500..1968067 (-) 2568 WP_255933101.1 penicillin-binding protein 1A -
  HB761_RS09205 (HB761_09190) pilM 1968246..1969262 (+) 1017 WP_255933102.1 type IV pilus biogenesis protein PilM Machinery gene
  HB761_RS09210 (HB761_09195) pilN 1969246..1969821 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB761_RS09215 (HB761_09200) pilO 1969814..1970407 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB761_RS09220 (HB761_09205) pilP 1970397..1970912 (+) 516 WP_010643498.1 pilus assembly protein PilP Machinery gene
  HB761_RS09225 (HB761_09210) pilQ 1970947..1972701 (+) 1755 WP_255940587.1 type IV pilus secretin PilQ Machinery gene
  HB761_RS09230 (HB761_09215) aroK 1972890..1973408 (+) 519 WP_010443904.1 shikimate kinase AroK -
  HB761_RS09235 (HB761_09220) aroB 1973440..1974540 (+) 1101 WP_255933103.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23002.49 Da        Isoelectric Point: 4.8135

>NTDB_id=433057 HB761_RS09215 WP_005432718.1 1969814..1970407(+) (pilO) [Vibrio campbellii strain LJC014]
MVNWQDLEMDEIAEWPLVPQLVAILLMVVLIQGFGYWFYLKPEEARLNTLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKESEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRANTYQFKPEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=433057 HB761_RS09215 WP_005432718.1 1969814..1970407(+) (pilO) [Vibrio campbellii strain LJC014]
ATGGTTAATTGGCAAGACCTAGAAATGGATGAAATCGCTGAATGGCCGCTGGTACCTCAGTTGGTGGCGATTCTTCTTAT
GGTGGTGTTGATCCAAGGTTTTGGTTACTGGTTTTATTTGAAGCCAGAGGAAGCTCGCCTTAATACCTTGATCCAAGAAG
AGCAGACTTTAAAAGTCGCGCTGAGGATCAAAGCCAATAAAGTAGCTGCACTGCCGCAGCTTCAGTCACAACTTGATGAA
CTGACCACCCGCTATGAACTGCTTTTGCAACAACTGCCGGCACAGAAAGAATTGGCGACCATGCTAGCGTCGGTTAATGA
ACTCGGCATCGATAACAAGCTGACATTTACGCGTATTGATTGGGGAGATAAAGAGTCAGAGCACTTCCTTTACCGTTTGC
CGCTCAATATTGAGCTAACGGGTGACTTCCATGACATCGGTCGTTTTTCACAGGCCATCGCCACCTTACCACGCATCATC
ACATTTCAAGATGTAACTTGGCAGCGAGTCAGTCAAGAGAGTGAAACCTTGCACTTCCGTGTAAGAGCAAACACTTACCA
ATTCAAACCAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A812GEL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

99.474

96.447

0.959
  pilO Vibrio cholerae strain A1552

72.251

96.954

0.701