Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HB761_RS09205 Genome accession   NZ_CP050467
Coordinates   1968246..1969262 (+) Length   338 a.a.
NCBI ID   WP_255933102.1    Uniprot ID   A0AAE9SMA2
Organism   Vibrio campbellii strain LJC014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1963246..1974262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB761_RS09190 (HB761_09175) - 1963610..1964338 (-) 729 WP_005432732.1 redoxin family protein -
  HB761_RS09195 (HB761_09180) oxyR 1964513..1965421 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  HB761_RS09200 (HB761_09185) - 1965500..1968067 (-) 2568 WP_255933101.1 penicillin-binding protein 1A -
  HB761_RS09205 (HB761_09190) pilM 1968246..1969262 (+) 1017 WP_255933102.1 type IV pilus biogenesis protein PilM Machinery gene
  HB761_RS09210 (HB761_09195) pilN 1969246..1969821 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB761_RS09215 (HB761_09200) pilO 1969814..1970407 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB761_RS09220 (HB761_09205) pilP 1970397..1970912 (+) 516 WP_010643498.1 pilus assembly protein PilP Machinery gene
  HB761_RS09225 (HB761_09210) pilQ 1970947..1972701 (+) 1755 WP_255940587.1 type IV pilus secretin PilQ Machinery gene
  HB761_RS09230 (HB761_09215) aroK 1972890..1973408 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37928.45 Da        Isoelectric Point: 6.2429

>NTDB_id=433055 HB761_RS09205 WP_255933102.1 1968246..1969262(+) (pilM) [Vibrio campbellii strain LJC014]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVVISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDMDEQMRGNDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGSHTTGCLFQQDPLADDAQVATEQFN
RQIVERIKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=433055 HB761_RS09205 WP_255933102.1 1968246..1969262(+) (pilM) [Vibrio campbellii strain LJC014]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGTCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGTAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAGTCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATATGGATGAGCAGATGCGAGGCAATGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACTCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGCCTGGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACGATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTTCTCACACTACGGGCTGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTATTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTCTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACCGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-216)

Type IV pilus assembly protein PilM; (PilM_2)

(238-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.337

100

0.973
  pilM Vibrio cholerae strain A1552

48.529

100

0.488