Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PARA_RS00535 Genome accession   NC_015964
Coordinates   102903..103346 (+) Length   147 a.a.
NCBI ID   WP_014064060.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae T3T1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97903..108346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PARA_RS00505 (PARA_01020) tadA 98117..98608 (-) 492 WP_050797950.1 tRNA adenosine(34) deaminase TadA -
  PARA_RS00510 (PARA_01030) - 98645..99496 (-) 852 WP_014064055.1 thymidylate synthase -
  PARA_RS00515 (PARA_01040) lgt 99507..100316 (-) 810 WP_014064056.1 prolipoprotein diacylglyceryl transferase -
  PARA_RS00520 (PARA_01050) - 100325..101119 (-) 795 WP_014064057.1 sulfite exporter TauE/SafE family protein -
  PARA_RS00525 (PARA_01060) rppH 101119..101712 (-) 594 WP_014064058.1 RNA pyrophosphohydrolase -
  PARA_RS00530 (PARA_01070) ampD 102197..102760 (-) 564 WP_014064059.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PARA_RS00535 (PARA_01080) pilA 102903..103346 (+) 444 WP_014064060.1 prepilin peptidase-dependent pilin Machinery gene
  PARA_RS00540 (PARA_01090) pilB 103353..104732 (+) 1380 WP_014064061.1 GspE/PulE family protein Machinery gene
  PARA_RS00545 (PARA_01100) pilC 104741..105964 (+) 1224 WP_014064062.1 type II secretion system F family protein Machinery gene
  PARA_RS00550 (PARA_01110) pilD 105961..106638 (+) 678 WP_014064063.1 prepilin peptidase Machinery gene
  PARA_RS00555 (PARA_01120) coaE 106727..107347 (+) 621 WP_014064064.1 dephospho-CoA kinase -
  PARA_RS00560 (PARA_01130) yacG 107340..107546 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  PARA_RS00565 (PARA_01140) - 107543..107824 (+) 282 WP_014064065.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15141.38 Da        Isoelectric Point: 8.4674

>NTDB_id=42224 PARA_RS00535 WP_014064060.1 102903..103346(+) (pilA) [Haemophilus parainfluenzae T3T1]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASTPYKSDVELCVYSTNAPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSSAK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=42224 PARA_RS00535 WP_014064060.1 102903..103346(+) (pilA) [Haemophilus parainfluenzae T3T1]
ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATCTCCGAATTGTTGCAAGCATCTACAC
CTTATAAATCTGATGTGGAGTTATGTGTCTATAGCACCAATGCCCCGACAAACTGTTCAGGTGGTTCAAATGGCATTGCA
GCAGACATCACTACTGCAAAAGGCTATGTCAAATCCATTACCACAAAATCAGGTGTGATTACGGTGACAGGAAATGGTGC
ATTGGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTCACCTGGACAACTGCTTGCCCAAGCA
ATGCAGATTTATTCCCTGCGGGTTTCTGCTCTTCAGCTAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

74.658

99.32

0.742
  pilA Haemophilus influenzae Rd KW20

72.603

99.32

0.721
  pilA Glaesserella parasuis strain SC1401

55.405

100

0.558
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA2 Legionella pneumophila str. Paris

42.188

87.075

0.367
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.526

100

0.367
  pilA Vibrio cholerae strain A1552

35.526

100

0.367
  pilA Vibrio cholerae C6706

35.526

100

0.367

Multiple sequence alignment