Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PARA_RS00540 Genome accession   NC_015964
Coordinates   103353..104732 (+) Length   459 a.a.
NCBI ID   WP_014064061.1    Uniprot ID   A0AB33QHN1
Organism   Haemophilus parainfluenzae T3T1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98353..109732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PARA_RS00510 (PARA_01030) - 98645..99496 (-) 852 WP_014064055.1 thymidylate synthase -
  PARA_RS00515 (PARA_01040) lgt 99507..100316 (-) 810 WP_014064056.1 prolipoprotein diacylglyceryl transferase -
  PARA_RS00520 (PARA_01050) - 100325..101119 (-) 795 WP_014064057.1 sulfite exporter TauE/SafE family protein -
  PARA_RS00525 (PARA_01060) rppH 101119..101712 (-) 594 WP_014064058.1 RNA pyrophosphohydrolase -
  PARA_RS00530 (PARA_01070) ampD 102197..102760 (-) 564 WP_014064059.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PARA_RS00535 (PARA_01080) pilA 102903..103346 (+) 444 WP_014064060.1 prepilin peptidase-dependent pilin Machinery gene
  PARA_RS00540 (PARA_01090) pilB 103353..104732 (+) 1380 WP_014064061.1 GspE/PulE family protein Machinery gene
  PARA_RS00545 (PARA_01100) pilC 104741..105964 (+) 1224 WP_014064062.1 type II secretion system F family protein Machinery gene
  PARA_RS00550 (PARA_01110) pilD 105961..106638 (+) 678 WP_014064063.1 prepilin peptidase Machinery gene
  PARA_RS00555 (PARA_01120) coaE 106727..107347 (+) 621 WP_014064064.1 dephospho-CoA kinase -
  PARA_RS00560 (PARA_01130) yacG 107340..107546 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  PARA_RS00565 (PARA_01140) - 107543..107824 (+) 282 WP_014064065.1 GNAT family N-acetyltransferase -
  PARA_RS00570 (PARA_01150) rhlB 107914..109173 (+) 1260 WP_014064066.1 ATP-dependent RNA helicase RhlB -
  PARA_RS00575 (PARA_01160) mreD 109214..109702 (-) 489 WP_014064067.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 51933.20 Da        Isoelectric Point: 6.1121

>NTDB_id=42225 PARA_RS00540 WP_014064061.1 103353..104732(+) (pilB) [Haemophilus parainfluenzae T3T1]
MAYQAIAKNGEIYHISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETAELK
QLLQSLAPKANQIEEQTTFYHHSEDESTANLSNDDEPVIQLLNGIFETALQKNASDIHLETQPSGLLIRLRIDGVLQPQP
IISKALANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
GNSCDCHQGYQGRIGVYQFLQCENNRYQTDFDSLYQSALEKVKDHITDLAEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=42225 PARA_RS00540 WP_014064061.1 103353..104732(+) (pilB) [Haemophilus parainfluenzae T3T1]
ATGGCTTATCAAGCAATCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCATTGCTGTTGCGTTACTTTGCGCTCCCTCTTAAAGAAGATGAACACCATTTATGGCTTGCAGTGGATTCACTCAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTCGAACCGATTTTATTTGAAACGGCCGAACTCAAA
CAACTTTTACAATCCCTTGCGCCTAAAGCCAATCAAATCGAAGAGCAAACGACGTTTTATCATCATTCAGAAGACGAAAG
CACTGCCAATTTATCTAATGATGATGAGCCCGTTATTCAATTATTAAATGGTATTTTTGAAACAGCCCTGCAAAAAAATG
CCTCAGACATTCATTTAGAAACTCAGCCAAGTGGGCTATTAATTCGTTTACGTATTGATGGTGTGCTCCAACCGCAACCG
ATTATTAGTAAAGCGCTCGCCAATCGTGTAATTTCTCGCCTCAAACTCTTAGCCAAACTGGATATTAGTGAAACTCGATT
ACCACAAGATGGCCGCTTTCAATTCAAAACCACATTTTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCTACGCATT
GGGGAGAAAAAGCAGTATTACGATTACAACAAAATAAACCTGTCCAACTAAGTTTTGCTGAGTTGGGTATGACACAAAAC
CAACAAAGTCAATTTCAACATGCGCTCAGCCAACCGCAAGGTTTAATCCTAGTGACAGGCCCAACGGGAAGTGGAAAAAG
TATCTCGCTTTATACCGCACTTCAATGGCTCAACACACCAGATAAACACATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGCATCATTCAAACTCAAGTAAATCCGCAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTAATGGCATTAAGAGCCGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCTATTTCACGCTTACAACAACTAGGTATTCAATCTC
ACGAAATTGAAAACAGTCTCTTATTAGTTATCGCACAACGATTGGTACGCAAACGTTGCTTAAAGTGCGGTCAACATTTC
GGTAATTCTTGTGATTGCCATCAGGGGTATCAAGGCCGTATTGGGGTATATCAATTTTTACAATGCGAAAACAACCGTTA
CCAAACTGATTTCGATTCACTTTATCAAAGCGCGTTAGAAAAAGTGAAAGATCACATCACTGATCTCGCCGAAATTCAAC
GCGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.54

98.475

0.773
  pilB Haemophilus influenzae Rd KW20

77.363

99.129

0.767
  pilB Glaesserella parasuis strain SC1401

59.956

98.475

0.59
  pilB Vibrio cholerae strain A1552

41.667

100

0.436
  pilB Vibrio campbellii strain DS40M4

41.322

100

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

41.25

100

0.431
  pilB Legionella pneumophila strain ERS1305867

39.059

100

0.416
  pilB Acinetobacter baylyi ADP1

38.229

100

0.414
  pilB Acinetobacter baumannii D1279779

38.525

100

0.41
  pilF Neisseria gonorrhoeae MS11

36.402

100

0.379
  pilF Thermus thermophilus HB27

38.695

93.464

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.095

100

0.362

Multiple sequence alignment