Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   GU337_RS02135 Genome accession   NZ_CP047614
Coordinates   417920..418372 (+) Length   150 a.a.
NCBI ID   WP_167839735.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain Lr_19_7     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 404367..447187 417920..418372 within 0


Gene organization within MGE regions


Location: 404367..447187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU337_RS02030 (GU337_02030) obgE 404367..405686 (+) 1320 WP_096039988.1 GTPase ObgE -
  GU337_RS02035 - 405773..405949 (+) 177 WP_003137484.1 hypothetical protein -
  GU337_RS02040 (GU337_02035) - 405946..406932 (+) 987 WP_061773784.1 PhoH family protein -
  GU337_RS02045 (GU337_02040) - 406925..407383 (+) 459 WP_138492264.1 hypothetical protein -
  GU337_RS02050 (GU337_02045) ybeY 407441..407932 (+) 492 WP_061773786.1 rRNA maturation RNase YbeY -
  GU337_RS02055 (GU337_02050) - 407916..408317 (+) 402 WP_138492265.1 diacylglycerol kinase family protein -
  GU337_RS02060 (GU337_02055) comFC 408320..408649 (-) 330 WP_163604800.1 phosphoribosyltransferase family protein Machinery gene
  GU337_RS02065 (GU337_02060) comFA/cflA 408964..410238 (-) 1275 WP_167839723.1 DEAD/DEAH box helicase Machinery gene
  GU337_RS02070 (GU337_02065) - 410282..410905 (+) 624 WP_096039992.1 YigZ family protein -
  GU337_RS02080 (GU337_02075) - 411474..412556 (-) 1083 WP_167839724.1 site-specific integrase -
  GU337_RS02085 (GU337_02080) - 412679..413230 (-) 552 WP_167839725.1 Ltp family lipoprotein -
  GU337_RS02090 (GU337_02085) - 413329..414030 (-) 702 WP_167839726.1 XRE family transcriptional regulator -
  GU337_RS02095 (GU337_02090) - 414224..414451 (+) 228 WP_167839727.1 hypothetical protein -
  GU337_RS02100 (GU337_02095) - 414474..415250 (+) 777 WP_167839728.1 phage antirepressor -
  GU337_RS02105 (GU337_02100) - 415316..415603 (+) 288 WP_167839729.1 helix-turn-helix domain-containing protein -
  GU337_RS02110 (GU337_02105) - 415859..416341 (+) 483 WP_167839730.1 hypothetical protein -
  GU337_RS02115 (GU337_02110) - 416334..416672 (+) 339 WP_167839731.1 hypothetical protein -
  GU337_RS02120 (GU337_02115) - 416665..416925 (+) 261 WP_167839732.1 hypothetical protein -
  GU337_RS02125 (GU337_02120) - 416927..417328 (+) 402 WP_167839733.1 hypothetical protein -
  GU337_RS02130 (GU337_02125) - 417340..417915 (+) 576 WP_167839734.1 ERF family protein -
  GU337_RS02135 (GU337_02130) ssbA 417920..418372 (+) 453 WP_167839735.1 single-stranded DNA-binding protein Machinery gene
  GU337_RS02140 (GU337_02135) - 418384..418539 (+) 156 WP_167839736.1 hypothetical protein -
  GU337_RS02145 (GU337_02140) - 418541..419287 (+) 747 WP_167839737.1 conserved phage C-terminal domain-containing protein -
  GU337_RS02150 (GU337_02145) - 419299..419691 (+) 393 WP_167839738.1 hypothetical protein -
  GU337_RS02155 (GU337_02150) - 419704..420582 (+) 879 WP_167839739.1 ATP-binding protein -
  GU337_RS02160 (GU337_02155) - 420591..421022 (+) 432 WP_167839740.1 DUF3850 domain-containing protein -
  GU337_RS02165 (GU337_02160) - 421019..421423 (+) 405 WP_167839741.1 RusA family crossover junction endodeoxyribonuclease -
  GU337_RS02170 (GU337_02165) - 421413..421688 (+) 276 WP_167839742.1 hypothetical protein -
  GU337_RS02175 (GU337_02170) - 421693..422088 (+) 396 WP_167839743.1 hypothetical protein -
  GU337_RS02180 (GU337_02175) - 422072..422470 (+) 399 WP_167839744.1 YopX family protein -
  GU337_RS02185 (GU337_02180) - 422474..422800 (+) 327 WP_167839745.1 hypothetical protein -
  GU337_RS02190 (GU337_02185) - 422793..423011 (+) 219 WP_167839746.1 hypothetical protein -
  GU337_RS02195 (GU337_02190) - 423008..423133 (+) 126 WP_167839747.1 DUF1660 family phage protein -
  GU337_RS02200 (GU337_02195) - 423146..423691 (+) 546 WP_167839748.1 DUF1642 domain-containing protein -
  GU337_RS02205 (GU337_02200) - 423727..423873 (+) 147 WP_167839749.1 hypothetical protein -
  GU337_RS02210 (GU337_02205) - 424122..424607 (+) 486 WP_167839750.1 class I SAM-dependent methyltransferase -
  GU337_RS02215 (GU337_02210) - 424856..425065 (+) 210 WP_167839751.1 hypothetical protein -
  GU337_RS02220 (GU337_02215) - 425072..425440 (+) 369 WP_167839550.1 hypothetical protein -
  GU337_RS02225 (GU337_02220) - 425476..425877 (+) 402 WP_167839752.1 ArpU family phage packaging/lysis transcriptional regulator -
  GU337_RS02240 (GU337_02235) - 427119..427367 (+) 249 WP_167839753.1 hypothetical protein -
  GU337_RS02245 (GU337_02240) - 427376..427531 (+) 156 WP_167839754.1 hypothetical protein -
  GU337_RS02250 (GU337_02245) - 427589..427906 (+) 318 WP_167839755.1 HNH endonuclease -
  GU337_RS02255 (GU337_02250) - 428003..428347 (+) 345 WP_167839756.1 P27 family phage terminase small subunit -
  GU337_RS02260 (GU337_02255) - 428360..430165 (+) 1806 WP_167839757.1 terminase large subunit -
  GU337_RS02265 (GU337_02260) - 430175..431371 (+) 1197 WP_167839758.1 phage portal protein -
  GU337_RS02270 (GU337_02265) - 431371..431973 (+) 603 WP_167839759.1 HK97 family phage prohead protease -
  GU337_RS02275 (GU337_02270) - 431936..433168 (+) 1233 WP_167839760.1 phage major capsid protein -
  GU337_RS02280 - 433180..433353 (+) 174 WP_167839761.1 hypothetical protein -
  GU337_RS02285 (GU337_02275) - 433346..433636 (+) 291 WP_167839762.1 hypothetical protein -
  GU337_RS02290 (GU337_02280) - 433629..433940 (+) 312 WP_167839763.1 phage head closure protein -
  GU337_RS02295 (GU337_02285) - 433940..434275 (+) 336 WP_167839764.1 HK97 gp10 family phage protein -
  GU337_RS02300 (GU337_02290) - 434272..434592 (+) 321 WP_167839765.1 hypothetical protein -
  GU337_RS02305 (GU337_02295) - 434604..435191 (+) 588 WP_167839766.1 major tail protein -
  GU337_RS02310 (GU337_02300) - 435259..435618 (+) 360 WP_167839767.1 hypothetical protein -
  GU337_RS02315 (GU337_02305) - 435933..438695 (+) 2763 WP_167839768.1 phage tail tape measure protein -
  GU337_RS02320 (GU337_02310) - 438699..439391 (+) 693 WP_167839769.1 phage tail domain-containing protein -
  GU337_RS02325 (GU337_02315) - 439388..443299 (+) 3912 WP_167839770.1 phage tail spike protein -
  GU337_RS02330 (GU337_02320) - 443446..443673 (+) 228 WP_167839771.1 hypothetical protein -
  GU337_RS02335 (GU337_02325) - 443689..444087 (+) 399 WP_167839772.1 phage holin family protein -
  GU337_RS02340 (GU337_02330) - 444176..444367 (+) 192 WP_167839773.1 hypothetical protein -
  GU337_RS02345 (GU337_02335) - 444357..445205 (+) 849 WP_167839774.1 peptidoglycan amidohydrolase family protein -
  GU337_RS02350 (GU337_02340) - 445366..445584 (+) 219 WP_167839775.1 hypothetical protein -
  GU337_RS02355 (GU337_02345) - 445642..445830 (+) 189 WP_167839776.1 hypothetical protein -
  GU337_RS02360 (GU337_02350) - 446324..447187 (+) 864 WP_167839777.1 S1 RNA-binding domain-containing protein -

Sequence


Protein


Download         Length: 150 a.a.        Molecular weight: 16634.40 Da        Isoelectric Point: 5.8631

>NTDB_id=414590 GU337_RS02135 WP_167839735.1 417920..418372(+) (ssbA) [Lactococcus raffinolactis strain Lr_19_7]
MINNTVLVGRVTKDIELKQTQANKSVTQFVLAVNRQFKNANGEREADFINIVAWGKTAELLAQYAHKGSQIGVVGRIQTR
NYENQQGQRVYVTEVVAESMTLLDSKGNNQNQQAQPQTQQSQNQTPDFGRNADPFSGGSSMTLSDDDLPF

Nucleotide


Download         Length: 453 bp        

>NTDB_id=414590 GU337_RS02135 WP_167839735.1 417920..418372(+) (ssbA) [Lactococcus raffinolactis strain Lr_19_7]
ATGATCAATAATACAGTACTGGTTGGACGAGTTACCAAAGACATCGAGTTAAAGCAGACGCAAGCTAATAAATCGGTCAC
TCAGTTTGTTTTGGCTGTCAATCGCCAGTTTAAAAACGCAAACGGAGAACGTGAAGCGGACTTTATCAATATTGTCGCAT
GGGGCAAAACTGCCGAACTTTTAGCGCAGTATGCGCATAAAGGATCACAAATTGGTGTAGTTGGTCGTATTCAAACGCGG
AACTACGAGAATCAGCAAGGTCAACGAGTTTATGTGACTGAGGTAGTCGCTGAAAGTATGACACTGCTTGATAGCAAAGG
CAACAACCAAAACCAACAGGCGCAACCGCAAACGCAGCAATCTCAAAATCAGACGCCTGATTTTGGCAGAAATGCTGATC
CGTTTTCTGGTGGTTCGTCAATGACTTTGAGCGATGACGATTTGCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

49.425

100

0.573

  ssb Latilactobacillus sakei subsp. sakei 23K

50

100

0.567

  ssbB/cilA Streptococcus mitis NCTC 12261

40.667

100

0.407

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.667

100

0.407

  ssbB/cilA Streptococcus mitis SK321

40

100

0.4

  ssbB/cilA Streptococcus pneumoniae Rx1

40

100

0.4

  ssbB/cilA Streptococcus pneumoniae D39

40

100

0.4

  ssbB/cilA Streptococcus pneumoniae R6

40

100

0.4

  ssbB Bacillus subtilis subsp. subtilis str. 168

55.66

70.667

0.393

  ssbA Streptococcus mutans UA159

38

100

0.38

  ssbB Lactococcus lactis subsp. cremoris KW2

47.863

78

0.373

  ssbB Streptococcus sobrinus strain NIDR 6715-7

52.336

71.333

0.373


Multiple sequence alignment