Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GQ674_RS01070 Genome accession   NZ_CP047135
Coordinates   233282..234415 (-) Length   377 a.a.
NCBI ID   WP_159495653.1    Uniprot ID   A0A6P1E8M1
Organism   Stenotrophomonas sp. 364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228282..239415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ674_RS01055 (GQ674_01055) - 229336..230739 (-) 1404 WP_159495651.1 DEAD/DEAH box helicase -
  GQ674_RS01060 (GQ674_01060) - 231459..232448 (+) 990 WP_159495652.1 fumarylacetoacetate hydrolase family protein -
  GQ674_RS01065 (GQ674_01065) maiA 232478..233122 (+) 645 WP_201290248.1 maleylacetoacetate isomerase -
  GQ674_RS01070 (GQ674_01070) pilU 233282..234415 (-) 1134 WP_159495653.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQ674_RS01075 (GQ674_01075) - 234570..234911 (+) 342 WP_051598900.1 DUF4398 domain-containing protein -
  GQ674_RS01080 (GQ674_01080) - 234911..235822 (+) 912 WP_201290202.1 hypothetical protein -
  GQ674_RS01085 (GQ674_01085) - 236216..236434 (+) 219 WP_038690653.1 YdcH family protein -
  GQ674_RS01090 (GQ674_01090) - 236636..238006 (+) 1371 WP_128095524.1 pyridoxal-phosphate dependent enzyme -
  GQ674_RS01095 (GQ674_01095) - 238137..239318 (+) 1182 WP_159495654.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41590.89 Da        Isoelectric Point: 6.2385

>NTDB_id=410913 GQ674_RS01070 WP_159495653.1 233282..234415(-) (pilU) [Stenotrophomonas sp. 364]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPEFERELELNMAIALAEAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVVSQRLVKGKDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRMAKNGIIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSANSTPAAITHGF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=410913 GQ674_RS01070 WP_159495653.1 233282..234415(-) (pilU) [Stenotrophomonas sp. 364]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCGTCGGACATGTTCTTGACCACCGGCGCCCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCGTTGGGGAACACCGGCTTGCCCCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCCGAGTTCGAGCGCGAGCTGGAACTGAACATGGCCATCGCCCTGGCCGAGGCCGGGCGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGTGCGATCCGCAGCAAGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTGGGGCTGGATACGCACGCCTTCCACAA
CGCACTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACCGCAACGTGCTGATGAACCTGTCGCTGAACCTGCGTGCGGTGGTGTCCCAGCG
CCTGGTGAAGGGCAAGGACGGGCGCCGCCTGCCGGCCACCGAGGTGTTGATCAACACCCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGGATGGCCAAGAACGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGGCTGGCGCTGAA
GTTCCGCCTCTCCGAAGGCGGCAGCGGCGAGCATGATCCGTACGCGGATTTCTCGGCCAACAGCACACCGGCCGCGATCA
CCCACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1E8M1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Acinetobacter baylyi ADP1

50.282

93.899

0.472
  pilU Vibrio cholerae strain A1552

48.611

95.491

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.652

99.204

0.443
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.716

89.655

0.374
  pilT Legionella pneumophila strain Lp02

41.716

89.655

0.374
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

41.176

90.186

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.655

0.369

Multiple sequence alignment