Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   GPZ88_RS05055 Genome accession   NZ_CP046919
Coordinates   970800..971741 (+) Length   313 a.a.
NCBI ID   WP_166043603.1    Uniprot ID   -
Organism   Streptococcus ruminicola strain CNU_G2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965800..976741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPZ88_RS05045 (GPZ88_05010) rpoC 966558..970196 (+) 3639 WP_166043599.1 DNA-directed RNA polymerase subunit beta' -
  GPZ88_RS05050 (GPZ88_05015) - 970362..970730 (+) 369 WP_166043601.1 DUF1033 family protein -
  GPZ88_RS05055 (GPZ88_05020) comYA 970800..971741 (+) 942 WP_166043603.1 competence type IV pilus ATPase ComGA Machinery gene
  GPZ88_RS05060 (GPZ88_05025) comYB 971665..972708 (+) 1044 WP_206282135.1 competence type IV pilus assembly protein ComGB Machinery gene
  GPZ88_RS05065 (GPZ88_05030) comGC/cglC 972708..973001 (+) 294 WP_074561132.1 competence type IV pilus major pilin ComGC Machinery gene
  GPZ88_RS05070 (GPZ88_05035) comYD 972985..973416 (+) 432 WP_157628644.1 competence type IV pilus minor pilin ComGD Machinery gene
  GPZ88_RS05075 (GPZ88_05040) comYE 973370..973663 (+) 294 WP_166043607.1 competence type IV pilus minor pilin ComGE Machinery gene
  GPZ88_RS05080 (GPZ88_05045) comYF 973647..974084 (+) 438 WP_133018089.1 competence type IV pilus minor pilin ComGF Machinery gene
  GPZ88_RS05085 (GPZ88_05050) comGG 974056..974406 (+) 351 WP_133018088.1 competence type IV pilus minor pilin ComGG -
  GPZ88_RS05090 (GPZ88_05055) comYH 974460..975416 (+) 957 WP_006531743.1 class I SAM-dependent methyltransferase Machinery gene
  GPZ88_RS05095 (GPZ88_05060) - 975470..976669 (+) 1200 WP_166043609.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35614.76 Da        Isoelectric Point: 5.4985

>NTDB_id=409426 GPZ88_RS05055 WP_166043603.1 970800..971741(+) (comYA) [Streptococcus ruminicola strain CNU_G2]
MIQEKAKQLIKDAVEKNAQDIYIVPKKEHYEIYQRVGDERQFIQELASDEMTSVISHFKFVAGMNVGEKRRSQLGSCDYE
FAEGEVISLRLSTVGDYRGRESLVIRLLYSGRHDLQYWFDGMKNILEAIDSRGLYLFSGPVGSGKTTLMYQLVREKFPDK
QVITIEDPVEIKQDNMLQLQLNDEIGMTYDNLIKLSLRHRPDILIIGEIRDSETARAVIRASLTGVLVFSTIHAKSIPGV
YARLLELGVSKQELENSLRAIVYQRLIGGGGVVDFAKGDFEQYPPTRWNEQIESLARDGHISLEEAKIEKIAL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=409426 GPZ88_RS05055 WP_166043603.1 970800..971741(+) (comYA) [Streptococcus ruminicola strain CNU_G2]
ATGATTCAGGAAAAAGCAAAGCAGCTGATTAAGGATGCTGTTGAAAAGAATGCGCAAGATATTTACATTGTTCCTAAGAA
GGAGCACTATGAAATTTATCAACGTGTTGGTGATGAACGTCAGTTTATTCAGGAGCTTGCTAGTGATGAGATGACAAGTG
TGATTAGTCACTTTAAATTTGTAGCTGGGATGAATGTTGGTGAGAAACGTCGTAGTCAGCTAGGGTCTTGTGATTATGAA
TTTGCTGAGGGTGAAGTGATTTCACTGCGTTTATCTACTGTTGGTGATTATCGCGGGCGTGAGAGCCTGGTCATCAGACT
TCTTTATTCAGGTCGTCATGATTTGCAATATTGGTTCGATGGGATGAAGAATATTTTAGAGGCGATTGATAGTCGAGGAC
TTTACCTTTTTTCCGGGCCGGTAGGGAGTGGAAAGACTACTCTCATGTATCAATTGGTTCGTGAAAAGTTCCCTGATAAG
CAGGTCATTACGATAGAAGACCCTGTTGAGATTAAGCAGGATAATATGTTGCAGCTGCAGCTTAATGATGAGATTGGCAT
GACTTATGATAATCTTATCAAGCTCTCGCTTCGTCATAGACCTGATATTTTGATTATTGGTGAGATTCGTGATAGTGAGA
CAGCGCGTGCGGTTATTAGAGCTAGTCTAACAGGTGTGCTCGTCTTTTCAACCATTCATGCTAAGAGTATCCCGGGTGTC
TATGCAAGATTATTAGAATTGGGAGTTAGTAAGCAGGAACTTGAAAATAGTTTGCGAGCTATTGTTTATCAACGATTAAT
TGGCGGTGGAGGTGTTGTGGATTTTGCCAAAGGAGATTTTGAACAGTATCCCCCAACAAGGTGGAATGAGCAAATTGAAA
GCCTTGCTAGGGACGGACATATTAGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

71.704

99.361

0.712
  comYA Streptococcus mutans UA140

71.383

99.361

0.709
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.419

99.042

0.668
  comYA Streptococcus gordonii str. Challis substr. CH1

65.595

99.361

0.652
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.064

99.681

0.649
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.103

99.681

0.639
  comGA Lactococcus lactis subsp. cremoris KW2

54.952

100

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

43.431

87.54

0.38

Multiple sequence alignment