Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GPZ88_RS05060 Genome accession   NZ_CP046919
Coordinates   971665..972708 (+) Length   347 a.a.
NCBI ID   WP_206282135.1    Uniprot ID   -
Organism   Streptococcus ruminicola strain CNU_G2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 966665..977708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPZ88_RS05050 (GPZ88_05015) - 970362..970730 (+) 369 WP_166043601.1 DUF1033 family protein -
  GPZ88_RS05055 (GPZ88_05020) comYA 970800..971741 (+) 942 WP_166043603.1 competence type IV pilus ATPase ComGA Machinery gene
  GPZ88_RS05060 (GPZ88_05025) comYB 971665..972708 (+) 1044 WP_206282135.1 competence type IV pilus assembly protein ComGB Machinery gene
  GPZ88_RS05065 (GPZ88_05030) comGC/cglC 972708..973001 (+) 294 WP_074561132.1 competence type IV pilus major pilin ComGC Machinery gene
  GPZ88_RS05070 (GPZ88_05035) comYD 972985..973416 (+) 432 WP_157628644.1 competence type IV pilus minor pilin ComGD Machinery gene
  GPZ88_RS05075 (GPZ88_05040) comYE 973370..973663 (+) 294 WP_166043607.1 competence type IV pilus minor pilin ComGE Machinery gene
  GPZ88_RS05080 (GPZ88_05045) comYF 973647..974084 (+) 438 WP_133018089.1 competence type IV pilus minor pilin ComGF Machinery gene
  GPZ88_RS05085 (GPZ88_05050) comGG 974056..974406 (+) 351 WP_133018088.1 competence type IV pilus minor pilin ComGG -
  GPZ88_RS05090 (GPZ88_05055) comYH 974460..975416 (+) 957 WP_006531743.1 class I SAM-dependent methyltransferase Machinery gene
  GPZ88_RS05095 (GPZ88_05060) - 975470..976669 (+) 1200 WP_166043609.1 acetate kinase -
  GPZ88_RS05100 (GPZ88_05065) - 976829..977026 (+) 198 WP_074561137.1 helix-turn-helix transcriptional regulator -
  GPZ88_RS05105 (GPZ88_05070) - 977042..977242 (+) 201 WP_074965270.1 hypothetical protein -
  GPZ88_RS05110 (GPZ88_05075) - 977243..977698 (+) 456 WP_240915112.1 ABC transporter permease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39572.98 Da        Isoelectric Point: 9.6038

>NTDB_id=409427 GPZ88_RS05060 WP_206282135.1 971665..972708(+) (comYB) [Streptococcus ruminicola strain CNU_G2]
MSKLKALLGTDILALRKQKSKKLPFKKQQKVIQLFNNLFNSGFNLTEIVSFLRRSQLLAEVYVDSMQESLLSGASLADMM
AKLGFSDTIVTQIALADVHGNCQQSLLKIDGYLASMSVVRKKLIEVATYPLILLSFLILIMLGLKNYLLPQLENQNFATQ
IISHFPMIFLGGFALLALGILLGVLYAKRLSPIYLYSQLSRLPIFGRYVRLYLTAYYAREWGNLIGQGIELMEIVELMQR
QKSRLFQEIGKDMQEALLAGQSFHQKVLDYPFFLRELSLMIEYGEVKSKLGRELDIYAEETWQNFFSQLTQATQLIQPLV
FVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=409427 GPZ88_RS05060 WP_206282135.1 971665..972708(+) (comYB) [Streptococcus ruminicola strain CNU_G2]
ATGAGCAAATTGAAAGCCTTGCTAGGGACGGACATATTAGCCTTGAGGAAGCAAAAATCGAAAAAATTGCCCTTTAAAAA
ACAGCAGAAGGTGATTCAATTATTTAACAATCTCTTTAACAGTGGCTTTAATTTAACAGAAATTGTTTCTTTTTTGAGAC
GAAGTCAATTGTTGGCAGAAGTTTATGTGGATAGTATGCAAGAATCTTTGCTAAGCGGGGCAAGTTTGGCAGATATGATG
GCTAAATTAGGCTTTTCAGATACAATTGTCACTCAGATTGCTTTGGCAGATGTTCATGGAAATTGTCAGCAGAGTTTGTT
AAAAATTGATGGCTACTTGGCCAGTATGTCAGTTGTTCGAAAGAAATTGATTGAAGTGGCGACTTATCCTTTGATTTTGC
TCAGCTTTTTAATTCTGATTATGCTGGGCTTGAAAAATTATTTGCTACCGCAGTTGGAAAATCAAAATTTTGCGACTCAG
ATTATCAGCCATTTTCCTATGATTTTTTTAGGTGGCTTTGCTCTTTTAGCTTTAGGAATTCTGCTTGGGGTTCTGTATGC
CAAGCGCTTATCGCCAATTTATCTGTATAGTCAGTTGAGTCGTTTGCCAATCTTTGGGCGGTACGTGCGTCTTTATTTGA
CCGCCTATTATGCGCGTGAGTGGGGAAATCTTATCGGGCAAGGTATTGAGCTTATGGAGATTGTAGAGCTCATGCAGCGG
CAAAAATCACGCCTTTTTCAAGAAATTGGAAAGGATATGCAAGAAGCTTTGCTTGCAGGGCAATCTTTTCACCAAAAGGT
TTTAGATTATCCTTTTTTCTTGCGTGAGCTTAGTTTGATGATTGAGTATGGTGAAGTCAAATCAAAATTAGGTCGTGAGT
TAGATATTTATGCCGAAGAGACTTGGCAGAATTTCTTTAGTCAATTGACACAGGCAACGCAGCTGATTCAGCCTTTGGTC
TTTGTCTTTGTTGCTTTAATCATCGTTTTAATTTATGTGGCAATGCTTTTGCCAATGTATCAAAATATGGGAGGAAATTT
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

65.19

91.066

0.594
  comYB Streptococcus mutans UA159

65.19

91.066

0.594
  comYB Streptococcus gordonii str. Challis substr. CH1

58.529

97.983

0.573
  comGB/cglB Streptococcus mitis NCTC 12261

58.457

97.118

0.568
  comGB/cglB Streptococcus mitis SK321

57.567

97.118

0.559
  comGB/cglB Streptococcus pneumoniae Rx1

56.973

97.118

0.553
  comGB/cglB Streptococcus pneumoniae D39

56.973

97.118

0.553
  comGB/cglB Streptococcus pneumoniae R6

56.973

97.118

0.553
  comGB/cglB Streptococcus pneumoniae TIGR4

56.973

97.118

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

52.083

96.83

0.504

Multiple sequence alignment