Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPX94_RS05550 Genome accession   NZ_CP046848
Coordinates   1202391..1204112 (+) Length   573 a.a.
NCBI ID   WP_078926717.1    Uniprot ID   A0A1T4QY24
Organism   Vibrio cincinnatiensis strain F8054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197391..1209112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX94_RS05530 pilM 1199670..1200692 (+) 1023 WP_154115192.1 type IV pilus assembly protein PilM Machinery gene
  GPX94_RS05535 pilN 1200682..1201275 (+) 594 WP_154115193.1 PilN domain-containing protein Machinery gene
  GPX94_RS05540 pilO 1201268..1201858 (+) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX94_RS05545 pilP 1201848..1202366 (+) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  GPX94_RS05550 pilQ 1202391..1204112 (+) 1722 WP_078926717.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX94_RS05555 aroK 1204299..1204817 (+) 519 WP_078926716.1 shikimate kinase AroK -
  GPX94_RS05560 aroB 1204845..1205936 (+) 1092 WP_154115194.1 3-dehydroquinate synthase -
  GPX94_RS05565 - 1205958..1207421 (+) 1464 WP_154115195.1 AAA family ATPase -
  GPX94_RS05570 - 1207491..1208312 (+) 822 WP_078926713.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPX94_RS05575 rpe 1208439..1209110 (+) 672 WP_078926712.1 ribulose-phosphate 3-epimerase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62783.13 Da        Isoelectric Point: 4.7265

>NTDB_id=408873 GPX94_RS05550 WP_078926717.1 1202391..1204112(+) (pilQ) [Vibrio cincinnatiensis strain F8054]
MIKGLNSVVVRSLRYLCIGVWTLSAVSVVASELTGANQLKNIDFRLNKNKEAVLIVELASPSTVVDVQKKAQGLTIELLN
TEVADDQLYLFDVKDFASAVETIEVFRQEPSTLLEVSIQGAFKYDYSLRSQYLEVVITELKKEEPAPLPNLMEKQGKLIS
INFQDIPVRNVLQLIADYNQFNLVVSDSVKGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQL
EKARLAEELGDLRSDIIKVHFAKASDIAEMIGGSGTVNMLSERGSISIDERTNALLIRELPENIKVIREIIESLDIPVKQ
VQIEARIVTVNEGNLDELGIRWGMSSKNGSHTVGGSIEGNMNTIGLYEPEDGTEMINNFLNVNLGATAANASSIAFQVAK
LGSGTLLDLELSAMQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLKVTPQITPDNRLVL
DLSVTQDRPGDVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSLTNTIDKVPLLGDLPFLGALFRRSYQQLGKS
ELLIFVTPQVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=408873 GPX94_RS05550 WP_078926717.1 1202391..1204112(+) (pilQ) [Vibrio cincinnatiensis strain F8054]
ATGATTAAAGGATTAAATTCGGTAGTGGTACGTAGTTTGCGTTACCTGTGTATAGGCGTGTGGACACTGTCGGCTGTCAG
CGTTGTTGCCAGTGAATTAACAGGAGCGAATCAATTAAAGAATATTGATTTTCGTTTAAATAAAAATAAAGAGGCGGTTT
TGATCGTCGAGTTGGCCTCTCCCTCAACGGTAGTGGACGTACAAAAAAAAGCTCAAGGATTAACGATTGAACTACTCAAT
ACGGAAGTTGCTGATGATCAGCTCTACTTATTCGATGTAAAAGATTTTGCTTCAGCGGTGGAAACGATTGAAGTGTTTCG
TCAAGAGCCAAGTACTTTGCTCGAAGTGAGTATTCAAGGGGCCTTTAAGTATGATTATTCTTTACGCAGCCAATATTTAG
AGGTGGTCATTACCGAACTAAAGAAGGAAGAACCCGCCCCCCTTCCTAATTTGATGGAAAAACAAGGTAAGCTTATCTCC
ATTAACTTCCAAGATATTCCTGTTCGAAATGTCTTACAGTTAATTGCGGATTACAACCAATTTAATTTAGTGGTTTCTGA
CTCAGTTAAAGGGAACTTAACCCTACGTTTGGATGGTGTTCCTTGGCAGCAGGTGCTTGATATTATTTTACAGGTCAAAG
GGCTTGATAAGCGCGTTGATGGAAACGTGATTCTCGTTGCACCGAAAGAGGAGTTGGATTTACGAGAAAAGCAACAACTC
GAGAAAGCGCGTTTAGCGGAAGAACTGGGTGATTTACGCTCGGACATCATCAAAGTACATTTTGCGAAAGCCTCGGATAT
TGCTGAGATGATAGGTGGAAGTGGAACTGTCAATATGTTATCAGAGCGTGGTTCTATTAGTATTGATGAGCGAACGAATG
CCCTCTTGATCCGCGAATTACCTGAAAACATTAAAGTGATTCGTGAAATCATTGAGTCACTGGATATTCCTGTCAAACAA
GTACAGATTGAAGCCAGAATTGTGACGGTGAATGAAGGCAACTTAGACGAATTAGGGATCCGGTGGGGGATGTCATCGAA
AAACGGCAGTCATACCGTGGGCGGTTCTATTGAAGGAAACATGAACACGATTGGCTTATATGAACCGGAAGATGGTACCG
AAATGATCAATAACTTCTTGAACGTTAACTTGGGGGCCACGGCTGCTAACGCCTCAAGCATTGCGTTTCAGGTCGCAAAA
TTGGGGTCAGGGACTTTGCTGGATCTTGAATTATCAGCGATGCAGCGCGAGTCTAAAGCTGAAATCATCTCGAGCCCACG
GTTGATCACCACCAATAAAAAACCCGCTTACATTGAGCAAGGTACGGAAATCCCCTACTTAGAATCCTCTTCTAGCGGGG
CAACGACGGTGGCATTTAAAAAAGCGGTTCTCAGTTTGAAAGTGACACCTCAAATCACCCCAGATAACCGTTTGGTACTG
GATTTAAGTGTCACACAGGATCGCCCCGGTGATGTGGTTAAAACCGGCACAGGGGAAGCGGTAGCGATTAATACTCAACG
GATTGGTACCCAAGTGTTAGTCAATAATGGAGAAACCGTTGTACTGGGTGGTATTTTCCAACATAGCTTGACTAATACCA
TAGATAAAGTGCCTTTACTGGGTGATCTCCCTTTTCTTGGAGCACTCTTTCGCCGTAGTTATCAGCAATTGGGTAAAAGT
GAATTGCTGATTTTTGTCACGCCTCAGGTGGTGATTCAATGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-320)

Bacterial type II and III secretion system protein (Secretin)

(413-571)

Secretin and TonB N terminus short domain (STN)

(181-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4QY24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.487

100

0.775
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.487

100

0.775
  pilQ Vibrio campbellii strain DS40M4

76.588

96.161

0.736
  pilQ Legionella pneumophila strain Lp02

42.308

95.288

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.308

95.288

0.403
  comQ Acinetobacter baylyi ADP1

38.66

100

0.393
  pilQ Pseudomonas aeruginosa PAK

39.818

95.986

0.382
  pilQ Acinetobacter baumannii strain A118

36.441

100

0.375
  pilQ Acinetobacter baumannii D1279779

36.441

100

0.375

Multiple sequence alignment