Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX94_RS05530 Genome accession   NZ_CP046848
Coordinates   1199670..1200692 (+) Length   340 a.a.
NCBI ID   WP_154115192.1    Uniprot ID   -
Organism   Vibrio cincinnatiensis strain F8054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1194670..1205692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX94_RS05510 - 1194869..1195138 (-) 270 WP_078926725.1 DUF3624 domain-containing protein -
  GPX94_RS05515 - 1195234..1195965 (-) 732 WP_154115190.1 glutathione peroxidase -
  GPX94_RS05520 oxyR 1196111..1197001 (+) 891 WP_078926723.1 DNA-binding transcriptional regulator OxyR -
  GPX94_RS05525 - 1197051..1199528 (-) 2478 WP_154115191.1 PBP1A family penicillin-binding protein -
  GPX94_RS05530 pilM 1199670..1200692 (+) 1023 WP_154115192.1 type IV pilus assembly protein PilM Machinery gene
  GPX94_RS05535 pilN 1200682..1201275 (+) 594 WP_154115193.1 PilN domain-containing protein Machinery gene
  GPX94_RS05540 pilO 1201268..1201858 (+) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX94_RS05545 pilP 1201848..1202366 (+) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  GPX94_RS05550 pilQ 1202391..1204112 (+) 1722 WP_078926717.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX94_RS05555 aroK 1204299..1204817 (+) 519 WP_078926716.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37908.17 Da        Isoelectric Point: 6.9695

>NTDB_id=408869 GPX94_RS05530 WP_154115192.1 1199670..1200692(+) (pilM) [Vibrio cincinnatiensis strain F8054]
MGKSLVTGVDIGHHSIKAVVLKLSGDTYSLVSYKEIAVTGDIFADNYTLDYQKIVKKLKELKKSLPLFNRKVSIAVPDNS
VISKILQIESDLEPAEQEFAIYQAFSHQSPLPIDDLSLDFIKISDNVLGRSRSATYQVYATRREVVESRISASEKAGLTP
VLLDVQMHSLVRIWQQVSQIQQRTNWMLLDIGYSQSSLCLDFIDKAPFCKEIPLGTRWFNQTEQPEGSHDVASLDSSTDV
QAFNQEFVERLARQIQLFTSVHGTHSLAGIWLSGGGASISGLAEAISERLALPCERFTPLSIFSHKLNKRRRTLNEQQRF
STAAGLALRGIEWLESGHAA

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=408869 GPX94_RS05530 WP_154115192.1 1199670..1200692(+) (pilM) [Vibrio cincinnatiensis strain F8054]
ATGGGTAAATCATTAGTTACAGGTGTTGATATTGGGCATCACAGCATTAAAGCTGTGGTTTTAAAGTTAAGTGGCGATAC
CTATTCGCTCGTTAGCTATAAAGAAATTGCCGTAACCGGCGACATTTTCGCCGATAACTACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTCAAAAAGAGCCTACCTTTGTTTAACCGCAAGGTCTCTATTGCCGTACCTGATAACTCG
GTCATCAGCAAAATATTGCAAATAGAAAGTGATCTTGAACCCGCTGAGCAAGAATTTGCTATTTATCAGGCGTTCTCTCA
TCAGTCACCATTACCGATTGACGATCTGAGTTTAGATTTCATTAAGATATCAGATAACGTACTGGGACGAAGTCGTAGTG
CAACGTATCAAGTCTATGCTACGCGCCGTGAAGTGGTGGAAAGCCGTATTTCTGCTTCTGAAAAGGCAGGCCTTACTCCA
GTACTTCTTGATGTACAGATGCACAGTTTAGTGCGTATCTGGCAGCAAGTGAGCCAAATTCAGCAGAGAACGAATTGGAT
GTTACTTGATATTGGCTATAGCCAAAGTTCTTTGTGCCTTGATTTCATCGATAAAGCCCCATTTTGTAAAGAGATACCAC
TGGGTACTCGTTGGTTTAACCAAACTGAGCAGCCAGAGGGAAGCCATGATGTTGCATCGTTGGACAGCTCAACCGATGTA
CAAGCCTTTAATCAAGAGTTTGTTGAACGATTGGCGCGGCAAATTCAGCTATTTACTTCCGTACATGGCACACACAGTTT
GGCGGGAATATGGCTTTCTGGAGGGGGAGCTTCTATCTCCGGATTAGCGGAGGCGATTAGTGAGCGACTCGCATTACCAT
GTGAGCGGTTTACACCGCTCTCTATCTTTAGTCATAAATTGAATAAGCGTCGGCGAACGTTAAATGAGCAGCAACGATTC
AGTACCGCTGCTGGGTTAGCGTTACGAGGTATTGAATGGTTGGAGAGTGGGCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

62.647

100

0.626
  pilM Vibrio campbellii strain DS40M4

48.529

100

0.485

Multiple sequence alignment