Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPX87_RS04930 Genome accession   NZ_CP046815
Coordinates   1043370..1045091 (-) Length   573 a.a.
NCBI ID   WP_078926717.1    Uniprot ID   A0A1T4QY24
Organism   Vibrio cincinnatiensis strain 1398-82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038370..1050091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX87_RS04905 rpe 1038372..1039043 (-) 672 WP_078926712.1 ribulose-phosphate 3-epimerase -
  GPX87_RS04910 - 1039170..1039991 (-) 822 WP_078926713.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPX87_RS04915 - 1040061..1041524 (-) 1464 WP_078926714.1 AAA family ATPase -
  GPX87_RS04920 aroB 1041546..1042637 (-) 1092 WP_078926715.1 3-dehydroquinate synthase -
  GPX87_RS04925 aroK 1042665..1043183 (-) 519 WP_078926716.1 shikimate kinase AroK -
  GPX87_RS04930 pilQ 1043370..1045091 (-) 1722 WP_078926717.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX87_RS04935 pilP 1045116..1045634 (-) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  GPX87_RS04940 pilO 1045624..1046214 (-) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX87_RS04945 pilN 1046207..1046800 (-) 594 WP_078926720.1 PilN domain-containing protein Machinery gene
  GPX87_RS04950 pilM 1046790..1047812 (-) 1023 WP_078926721.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62783.13 Da        Isoelectric Point: 4.7265

>NTDB_id=408261 GPX87_RS04930 WP_078926717.1 1043370..1045091(-) (pilQ) [Vibrio cincinnatiensis strain 1398-82]
MIKGLNSVVVRSLRYLCIGVWTLSAVSVVASELTGANQLKNIDFRLNKNKEAVLIVELASPSTVVDVQKKAQGLTIELLN
TEVADDQLYLFDVKDFASAVETIEVFRQEPSTLLEVSIQGAFKYDYSLRSQYLEVVITELKKEEPAPLPNLMEKQGKLIS
INFQDIPVRNVLQLIADYNQFNLVVSDSVKGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQL
EKARLAEELGDLRSDIIKVHFAKASDIAEMIGGSGTVNMLSERGSISIDERTNALLIRELPENIKVIREIIESLDIPVKQ
VQIEARIVTVNEGNLDELGIRWGMSSKNGSHTVGGSIEGNMNTIGLYEPEDGTEMINNFLNVNLGATAANASSIAFQVAK
LGSGTLLDLELSAMQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLKVTPQITPDNRLVL
DLSVTQDRPGDVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSLTNTIDKVPLLGDLPFLGALFRRSYQQLGKS
ELLIFVTPQVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=408261 GPX87_RS04930 WP_078926717.1 1043370..1045091(-) (pilQ) [Vibrio cincinnatiensis strain 1398-82]
ATGATTAAAGGATTAAATTCGGTAGTGGTACGTAGTTTGCGTTACCTGTGTATAGGCGTGTGGACACTGTCGGCTGTCAG
CGTTGTTGCCAGTGAATTAACAGGAGCGAATCAATTAAAGAATATTGATTTTCGTTTAAATAAAAATAAAGAGGCGGTTT
TGATCGTCGAGTTGGCCTCTCCCTCAACGGTAGTGGACGTACAAAAAAAAGCTCAAGGATTAACGATTGAACTACTCAAT
ACGGAAGTTGCTGATGATCAGCTCTACTTATTCGATGTAAAAGATTTTGCTTCAGCGGTGGAAACGATTGAAGTGTTTCG
TCAAGAGCCAAGTACTTTGCTCGAAGTGAGTATTCAAGGGGCCTTTAAGTATGATTATTCTTTACGCAGCCAATATTTAG
AGGTGGTCATTACCGAACTAAAGAAGGAAGAACCCGCCCCTCTTCCTAATTTGATGGAAAAACAAGGTAAGCTTATCTCC
ATTAACTTCCAAGATATTCCTGTTCGAAATGTCTTACAGTTAATTGCGGATTACAACCAATTTAATTTAGTGGTTTCTGA
CTCAGTTAAAGGGAACTTAACCCTACGTTTGGATGGTGTTCCTTGGCAGCAGGTGCTTGATATTATTTTACAGGTCAAAG
GGCTTGATAAGCGCGTTGATGGAAACGTGATTCTCGTTGCACCGAAAGAGGAGTTGGATTTACGAGAAAAGCAACAACTC
GAGAAAGCGCGTTTAGCGGAAGAACTGGGTGATTTACGCTCGGACATCATCAAAGTACATTTTGCGAAAGCCTCGGATAT
TGCTGAGATGATAGGTGGAAGTGGAACTGTCAATATGTTATCAGAGCGTGGTTCTATTAGTATTGATGAGCGAACGAATG
CCCTCTTGATCCGCGAATTACCTGAAAACATTAAAGTGATTCGTGAAATCATTGAGTCACTGGATATTCCTGTCAAACAA
GTACAGATTGAAGCCAGAATTGTGACGGTGAATGAAGGCAACTTAGACGAATTAGGGATCCGGTGGGGGATGTCATCGAA
AAACGGCAGTCATACCGTGGGCGGTTCTATTGAAGGAAACATGAACACGATTGGCTTATATGAGCCGGAAGATGGTACCG
AAATGATCAATAACTTCTTGAACGTTAACTTGGGGGCCACGGCTGCTAACGCCTCAAGCATTGCGTTTCAGGTCGCAAAA
TTGGGGTCAGGGACTTTGCTAGATCTTGAATTATCAGCGATGCAGCGCGAGTCTAAAGCTGAAATCATTTCGAGCCCACG
GTTGATCACCACCAATAAAAAACCCGCTTACATTGAGCAAGGTACGGAAATCCCCTACTTAGAATCTTCTTCTAGCGGGG
CAACGACGGTGGCATTTAAAAAAGCGGTTCTCAGCTTGAAAGTGACACCTCAAATCACCCCAGATAACCGTTTGGTACTG
GATTTAAGTGTCACACAGGATCGCCCTGGTGATGTGGTTAAAACCGGCACAGGGGAAGCGGTAGCGATTAATACTCAACG
GATTGGTACCCAAGTGTTGGTCAATAATGGAGAAACCGTTGTTCTGGGTGGTATTTTCCAACATAGCTTGACTAATACCA
TAGATAAAGTGCCTTTACTGGGTGATCTCCCTTTTCTTGGAGCACTCTTTCGCCGTAGTTATCAGCAATTGGGTAAAAGT
GAATTGCTGATTTTTGTCACGCCTCAGGTGGTGATTCAATGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-320)

Bacterial type II and III secretion system protein (Secretin)

(413-571)

Secretin and TonB N terminus short domain (STN)

(181-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4QY24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.487

100

0.775
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.487

100

0.775
  pilQ Vibrio campbellii strain DS40M4

76.588

96.161

0.736
  pilQ Legionella pneumophila strain Lp02

42.308

95.288

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.308

95.288

0.403
  comQ Acinetobacter baylyi ADP1

38.66

100

0.393
  pilQ Pseudomonas aeruginosa PAK

39.818

95.986

0.382
  pilQ Acinetobacter baumannii strain A118

36.441

100

0.375
  pilQ Acinetobacter baumannii D1279779

36.441

100

0.375

Multiple sequence alignment