Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX87_RS04950 Genome accession   NZ_CP046815
Coordinates   1046790..1047812 (-) Length   340 a.a.
NCBI ID   WP_078926721.1    Uniprot ID   A0A1T4QY68
Organism   Vibrio cincinnatiensis strain 1398-82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1041790..1052812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX87_RS04925 aroK 1042665..1043183 (-) 519 WP_078926716.1 shikimate kinase AroK -
  GPX87_RS04930 pilQ 1043370..1045091 (-) 1722 WP_078926717.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX87_RS04935 pilP 1045116..1045634 (-) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  GPX87_RS04940 pilO 1045624..1046214 (-) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX87_RS04945 pilN 1046207..1046800 (-) 594 WP_078926720.1 PilN domain-containing protein Machinery gene
  GPX87_RS04950 pilM 1046790..1047812 (-) 1023 WP_078926721.1 type IV pilus assembly protein PilM Machinery gene
  GPX87_RS04955 - 1047954..1050431 (+) 2478 WP_078926722.1 PBP1A family penicillin-binding protein -
  GPX87_RS04960 oxyR 1050481..1051371 (-) 891 WP_078926723.1 DNA-binding transcriptional regulator OxyR -
  GPX87_RS04965 - 1051517..1052248 (+) 732 WP_078926724.1 glutathione peroxidase -
  GPX87_RS04970 - 1052344..1052613 (+) 270 WP_078926725.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37911.13 Da        Isoelectric Point: 6.9695

>NTDB_id=408265 GPX87_RS04950 WP_078926721.1 1046790..1047812(-) (pilM) [Vibrio cincinnatiensis strain 1398-82]
MGKSLVTGVDIGHHSIKAVVLKLSGDTYSLVSYKEIAVTGDIFADNYTLDYQKIVKKLKELKKSLPLFNRKVSIAVPDNS
VISKILQIESDLEPAEQEFAIYQAFSHQSPLPIDDLSLDFIKISDNVLGRSRSATYQVYATRREVVESRISASEKAGLNP
VLLDVQMHSLVRIWQQVSQIQQRTNWMLLDIGYSQSSLCLDFIDKAPFCKEIPLGTRWFNQTEQSEGSHDVASLDSSTDV
QAFNQEFVERLARQIQLFTSVHGTHSLAGIWLSGGGASISGLAEAISERLALPCERFTPLSIFSHKLNKRRRTLNEQQRF
STAAGLALRGIEWLESGHAA

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=408265 GPX87_RS04950 WP_078926721.1 1046790..1047812(-) (pilM) [Vibrio cincinnatiensis strain 1398-82]
ATGGGTAAATCATTAGTTACAGGTGTTGATATTGGGCATCACAGCATTAAAGCTGTGGTTTTAAAGTTAAGTGGCGATAC
CTATTCGCTCGTTAGCTATAAAGAAATTGCCGTAACCGGCGACATTTTCGCCGATAACTACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTCAAAAAGAGCCTACCTTTGTTTAACCGCAAGGTCTCTATTGCCGTACCTGATAACTCG
GTCATCAGCAAAATATTGCAAATAGAAAGTGATCTTGAGCCCGCTGAGCAAGAATTTGCTATTTATCAGGCGTTCTCTCA
TCAGTCACCATTACCGATTGACGATCTGAGTTTAGATTTCATTAAGATATCAGATAACGTACTGGGACGAAGTCGTAGTG
CAACGTATCAAGTCTATGCTACGCGCCGTGAAGTGGTGGAAAGCCGTATTTCTGCTTCTGAAAAGGCAGGCCTTAATCCA
GTACTTCTTGATGTACAGATGCACAGTTTAGTGCGTATCTGGCAGCAAGTGAGCCAAATTCAGCAGAGAACGAATTGGAT
GTTACTTGATATTGGCTATAGCCAAAGTTCTTTGTGCCTTGATTTCATCGATAAAGCCCCATTTTGTAAAGAGATACCAC
TGGGTACTCGTTGGTTTAACCAAACTGAGCAGTCAGAGGGAAGTCATGATGTTGCATCGTTGGACAGCTCAACCGATGTA
CAAGCCTTTAATCAAGAGTTTGTTGAACGATTGGCGCGGCAAATTCAGCTATTTACTTCCGTACATGGCACACACAGTTT
GGCGGGAATATGGCTTTCTGGAGGGGGAGCTTCTATCTCCGGATTAGCGGAGGCGATTAGTGAGCGACTCGCATTACCAT
GTGAGCGGTTTACACCGCTCTCTATCTTTAGTCATAAATTGAATAAGCGTCGGCGAACGTTAAATGAGCAGCAACGATTC
AGTACCGCTGCTGGGTTAGCGTTACGAGGTATTGAATGGTTGGAGAGTGGGCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4QY68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

62.647

100

0.626
  pilM Vibrio campbellii strain DS40M4

48.824

100

0.488

Multiple sequence alignment