Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GPX87_RS04945 Genome accession   NZ_CP046815
Coordinates   1046207..1046800 (-) Length   197 a.a.
NCBI ID   WP_078926720.1    Uniprot ID   A0A1T4QYC1
Organism   Vibrio cincinnatiensis strain 1398-82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1041207..1051800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX87_RS04920 aroB 1041546..1042637 (-) 1092 WP_078926715.1 3-dehydroquinate synthase -
  GPX87_RS04925 aroK 1042665..1043183 (-) 519 WP_078926716.1 shikimate kinase AroK -
  GPX87_RS04930 pilQ 1043370..1045091 (-) 1722 WP_078926717.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX87_RS04935 pilP 1045116..1045634 (-) 519 WP_078926718.1 pilus assembly protein PilP Machinery gene
  GPX87_RS04940 pilO 1045624..1046214 (-) 591 WP_078926719.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX87_RS04945 pilN 1046207..1046800 (-) 594 WP_078926720.1 PilN domain-containing protein Machinery gene
  GPX87_RS04950 pilM 1046790..1047812 (-) 1023 WP_078926721.1 type IV pilus assembly protein PilM Machinery gene
  GPX87_RS04955 - 1047954..1050431 (+) 2478 WP_078926722.1 PBP1A family penicillin-binding protein -
  GPX87_RS04960 oxyR 1050481..1051371 (-) 891 WP_078926723.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22629.04 Da        Isoelectric Point: 9.7503

>NTDB_id=408264 GPX87_RS04945 WP_078926720.1 1046207..1046800(-) (pilN) [Vibrio cincinnatiensis strain 1398-82]
MLHNINLLPWREVQREAYRQHFIHLLILGVLLALGVQGVAGSYLEQQKQQQQGRLDFLNQHIRQLDERLHALKVTEQEHK
ALLTRLKVVEALQQKRNKSTDFMHLMPELIPEGVYVDKIKMNGQEVEMNGISDSTARLATMLDNLEKSPQLSAVGMHSIV
SGNRRFGKQFQSFKVSFLFHTSDQVVQKPAQKEASHD

Nucleotide


Download         Length: 594 bp        

>NTDB_id=408264 GPX87_RS04945 WP_078926720.1 1046207..1046800(-) (pilN) [Vibrio cincinnatiensis strain 1398-82]
ATGCTGCATAACATTAATTTGTTGCCGTGGCGAGAAGTACAGCGAGAAGCCTATCGGCAGCATTTCATCCATTTATTAAT
CTTAGGCGTACTTTTAGCGCTGGGCGTGCAAGGGGTGGCGGGAAGCTATCTAGAGCAGCAGAAGCAACAGCAGCAGGGGC
GACTGGATTTTCTTAACCAGCATATTCGCCAGTTAGATGAACGCCTTCATGCTTTAAAAGTGACAGAGCAAGAACATAAA
GCCTTATTAACCCGTTTAAAAGTGGTGGAGGCTTTGCAACAGAAACGCAATAAAAGTACTGACTTTATGCACCTGATGCC
GGAGTTAATTCCTGAAGGCGTCTATGTCGATAAAATCAAGATGAATGGGCAAGAGGTCGAAATGAACGGTATTAGCGATA
GTACTGCGCGTTTGGCGACCATGTTGGATAATTTGGAAAAATCGCCTCAGTTATCTGCAGTGGGAATGCACTCGATTGTG
TCGGGCAATCGTCGCTTTGGTAAGCAGTTTCAGAGTTTTAAAGTCTCTTTCCTCTTTCATACTTCCGATCAAGTGGTTCA
AAAGCCCGCTCAAAAGGAGGCTTCTCATGACTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(105-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4QYC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

67.232

89.848

0.604
  pilN Vibrio campbellii strain DS40M4

62.57

90.863

0.569

Multiple sequence alignment