Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPW66_RS09890 Genome accession   NZ_CP046736
Coordinates   1081845..1082270 (-) Length   141 a.a.
NCBI ID   WP_032473121.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076845..1087270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS09870 coaE 1077346..1077954 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  GPW66_RS09875 pilD 1077951..1078826 (-) 876 WP_000418753.1 A24 family peptidase Machinery gene
  GPW66_RS09880 pilC 1078884..1080110 (-) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  GPW66_RS09885 pilB 1080153..1081841 (-) 1689 WP_158106346.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW66_RS09890 pilA 1081845..1082270 (-) 426 WP_032473121.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW66_RS09895 nadC 1082515..1083405 (-) 891 WP_001888470.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW66_RS09900 ampD 1083703..1084248 (+) 546 WP_000567317.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW66_RS09905 fldB 1084333..1084851 (-) 519 WP_000690112.1 flavodoxin FldB -
  GPW66_RS09910 xerD 1084989..1085897 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GPW66_RS09915 dsbC 1085914..1086666 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14657.71 Da        Isoelectric Point: 8.9683

>NTDB_id=406835 GPW66_RS09890 WP_032473121.1 1081845..1082270(-) (pilA) [Vibrio cholerae strain 3528-08]
MKAYKNKQQKGFTLIELMIVVAIIGVLAAIAVPAYKDYVTKSELASGFATIKSVITPAELYFQENGKISASASTVDVLGV
SSGANTLGSLTIPSDNQIQFAHNSGAAAGARFVYSRDTSGWSCTYTQPTAVSAAAPKGCAQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=406835 GPW66_RS09890 WP_032473121.1 1081845..1082270(-) (pilA) [Vibrio cholerae strain 3528-08]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGCGTGTT
GGCAGCGATTGCTGTGCCTGCTTATAAGGATTATGTAACCAAGAGCGAATTGGCATCTGGTTTCGCTACTATCAAGTCCG
TGATAACTCCAGCTGAACTTTATTTCCAAGAAAATGGAAAAATAAGTGCAAGCGCAAGCACTGTTGACGTTCTCGGTGTA
TCTTCTGGCGCAAATACTTTGGGTTCTCTGACTATTCCGTCTGACAATCAAATTCAATTTGCACATAATTCAGGGGCTGC
GGCAGGTGCAAGATTTGTTTACTCAAGAGACACTAGTGGCTGGAGTTGTACTTATACTCAACCAACCGCTGTGAGTGCTG
CCGCGCCTAAAGGCTGTGCACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

48.052

100

0.525
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.857

100

0.468
  pilA Vibrio cholerae strain A1552

42.857

100

0.468
  pilA Vibrio cholerae C6706

42.857

100

0.468
  pilA Pseudomonas aeruginosa PAK

38.158

100

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.13

97.872

0.383
  pilA Acinetobacter baumannii strain A118

38.686

97.163

0.376
  pilA Ralstonia pseudosolanacearum GMI1000

33.974

100

0.376
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.695

92.908

0.369

Multiple sequence alignment