Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GPW66_RS09875 Genome accession   NZ_CP046736
Coordinates   1077951..1078826 (-) Length   291 a.a.
NCBI ID   WP_000418753.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072951..1083826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS09855 parC 1073901..1076186 (+) 2286 WP_032473120.1 DNA topoisomerase IV subunit A -
  GPW66_RS09860 yacG 1076235..1076432 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW66_RS09865 zapD 1076574..1077314 (-) 741 WP_000207194.1 cell division protein ZapD -
  GPW66_RS09870 coaE 1077346..1077954 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  GPW66_RS09875 pilD 1077951..1078826 (-) 876 WP_000418753.1 A24 family peptidase Machinery gene
  GPW66_RS09880 pilC 1078884..1080110 (-) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  GPW66_RS09885 pilB 1080153..1081841 (-) 1689 WP_158106346.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW66_RS09890 pilA 1081845..1082270 (-) 426 WP_032473121.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW66_RS09895 nadC 1082515..1083405 (-) 891 WP_001888470.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32419.67 Da        Isoelectric Point: 7.1618

>NTDB_id=406832 GPW66_RS09875 WP_000418753.1 1077951..1078826(-) (pilD) [Vibrio cholerae strain 3528-08]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFGVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=406832 GPW66_RS09875 WP_000418753.1 1077951..1078826(-) (pilD) [Vibrio cholerae strain 3528-08]
ATGGAACTGTTTTATTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCTACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATTCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTGTTTGGCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTATTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCTATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75.347

98.969

0.746
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443

Multiple sequence alignment