Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GPW66_RS09880 Genome accession   NZ_CP046736
Coordinates   1078884..1080110 (-) Length   408 a.a.
NCBI ID   WP_000648507.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073884..1085110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS09855 parC 1073901..1076186 (+) 2286 WP_032473120.1 DNA topoisomerase IV subunit A -
  GPW66_RS09860 yacG 1076235..1076432 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW66_RS09865 zapD 1076574..1077314 (-) 741 WP_000207194.1 cell division protein ZapD -
  GPW66_RS09870 coaE 1077346..1077954 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  GPW66_RS09875 pilD 1077951..1078826 (-) 876 WP_000418753.1 A24 family peptidase Machinery gene
  GPW66_RS09880 pilC 1078884..1080110 (-) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  GPW66_RS09885 pilB 1080153..1081841 (-) 1689 WP_158106346.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW66_RS09890 pilA 1081845..1082270 (-) 426 WP_032473121.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW66_RS09895 nadC 1082515..1083405 (-) 891 WP_001888470.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW66_RS09900 ampD 1083703..1084248 (+) 546 WP_000567317.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW66_RS09905 fldB 1084333..1084851 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45104.41 Da        Isoelectric Point: 10.3424

>NTDB_id=406833 GPW66_RS09880 WP_000648507.1 1078884..1080110(-) (pilC) [Vibrio cholerae strain 3528-08]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKGKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=406833 GPW66_RS09880 WP_000648507.1 1078884..1080110(-) (pilC) [Vibrio cholerae strain 3528-08]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAGGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAACAGCTACGCGCCAAGGTGATCAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTTGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCAGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTGAAAACCAGTCGCTTGGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGCACGCTTGCCACCAGCTTTGCTGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTATATTGCCATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTTGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.755

100

0.998
  pilC Vibrio campbellii strain DS40M4

74

98.039

0.725
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402

Multiple sequence alignment