Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPW66_RS09885 Genome accession   NZ_CP046736
Coordinates   1080153..1081841 (-) Length   562 a.a.
NCBI ID   WP_158106346.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075153..1086841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS09860 yacG 1076235..1076432 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW66_RS09865 zapD 1076574..1077314 (-) 741 WP_000207194.1 cell division protein ZapD -
  GPW66_RS09870 coaE 1077346..1077954 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  GPW66_RS09875 pilD 1077951..1078826 (-) 876 WP_000418753.1 A24 family peptidase Machinery gene
  GPW66_RS09880 pilC 1078884..1080110 (-) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  GPW66_RS09885 pilB 1080153..1081841 (-) 1689 WP_158106346.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW66_RS09890 pilA 1081845..1082270 (-) 426 WP_032473121.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW66_RS09895 nadC 1082515..1083405 (-) 891 WP_001888470.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPW66_RS09900 ampD 1083703..1084248 (+) 546 WP_000567317.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPW66_RS09905 fldB 1084333..1084851 (-) 519 WP_000690112.1 flavodoxin FldB -
  GPW66_RS09910 xerD 1084989..1085897 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GPW66_RS09915 dsbC 1085914..1086666 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62043.21 Da        Isoelectric Point: 5.9025

>NTDB_id=406834 GPW66_RS09885 WP_158106346.1 1080153..1081841(-) (pilB) [Vibrio cholerae strain 3528-08]
MLTNLVVILRQAELISATQEQAVVSQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQNQFAFQPNE
ILYEANTAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIELLAKANGMLSLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=406834 GPW66_RS09885 WP_158106346.1 1080153..1081841(-) (pilB) [Vibrio cholerae strain 3528-08]
ATGCTCACCAACCTTGTTGTTATCTTGCGTCAGGCTGAGTTAATCAGCGCGACGCAAGAACAAGCGGTGGTTAGCCAAGT
GAGCGCTTCGGGAACTTCGGTACCCGAAGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTGACTGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTCGGGCTGCGTGAA
CTAATAACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGATCGATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCACA
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTTGAAACTCAGCAACCGGCTAGCCATTTAAGTCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTACGCCAAGATCCGGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACAAACTCGGCCGCCGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAATCTGGCGTCATCCCTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCCGCGCTATTGCAAAACCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATACGGCGGGGTGCAACGAGTGTACGGGCGGCTATTCAGGTCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACTGAGCTGGCGGAGGCCATTATGCAACGCGCCAGTATTCATCAAATTGAACTTTTAGCCAAAGCCAATG
GTATGTTGAGCTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

97.865

100

0.979
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.31

100

0.733
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50

92.883

0.464
  pilF Neisseria gonorrhoeae MS11

46.099

100

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

36.86

100

0.397
  pilF Thermus thermophilus HB27

39.187

96.263

0.377
  pilB Haemophilus influenzae 86-028NP

39.006

93.06

0.363

Multiple sequence alignment