Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GOQ09_RS23720 Genome accession   NZ_CP046622
Coordinates   5084255..5085391 (+) Length   378 a.a.
NCBI ID   WP_157616144.1    Uniprot ID   -
Organism   Variovorax paradoxus strain CSUSB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5079255..5090391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOQ09_RS23705 (GOQ09_23695) - 5079540..5082227 (+) 2688 WP_157616142.1 hypothetical protein -
  GOQ09_RS23710 (GOQ09_23700) - 5082431..5083132 (-) 702 WP_157616143.1 YggS family pyridoxal phosphate-dependent enzyme -
  GOQ09_RS23715 (GOQ09_23705) pilT 5083184..5084227 (+) 1044 WP_021012685.1 type IV pilus twitching motility protein PilT Machinery gene
  GOQ09_RS23720 (GOQ09_23710) pilU 5084255..5085391 (+) 1137 WP_157616144.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GOQ09_RS23725 (GOQ09_23715) - 5085443..5086351 (+) 909 WP_157616145.1 NAD(P)-dependent oxidoreductase -
  GOQ09_RS23730 (GOQ09_23720) - 5086404..5087168 (-) 765 WP_157616146.1 BON domain-containing protein -
  GOQ09_RS23735 (GOQ09_23725) - 5087165..5087809 (-) 645 WP_157616147.1 SIS domain-containing protein -
  GOQ09_RS23740 (GOQ09_23730) - 5087901..5088266 (-) 366 WP_157616148.1 YraN family protein -
  GOQ09_RS23745 (GOQ09_23735) rsmI 5088312..5089271 (+) 960 WP_157616149.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  GOQ09_RS23750 (GOQ09_23740) - 5089274..5090002 (-) 729 WP_157616150.1 glutathione S-transferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41888.95 Da        Isoelectric Point: 6.4189

>NTDB_id=406123 GOQ09_RS23720 WP_157616144.1 5084255..5085391(+) (pilU) [Variovorax paradoxus strain CSUSB]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTIMVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLIPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=406123 GOQ09_RS23720 WP_157616144.1 5084255..5085391(+) (pilU) [Variovorax paradoxus strain CSUSB]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
TACGGCGGACTTTCCGCCCGCCATCAAGGTCGATGGCAAGGTGACCAAGGTGTCGCAGCAGGCGCTGGGGGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGGCAGACGGCGGAGTTCGAGCGCACCAAGGAGTGCAACTTTGCGATC
TCGCCGACCGGCATCGGCCGCTTTCGCGTGAATGCCTTCGTGCAGCAAGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCATGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCAATGATCGACTGGCGCAACGAAAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTGGAATTTGTGCACCCGCACAAGAACTGCGTGGTGACACAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCGGCGCTCAAGAACACGCTGCGCCAAGCGCCAGACGTCATCTTGATGGGCGAAATTCGCG
ACCGCGAAACCATGGAACACGCGGTGGCTTTTGCCGAAACCGGCCACCTTTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTTCCCGAAGAACGCCGCGCACAGCTGCTGATGGACTTGTCGCTGAACCT
GCGTTCGCTGATTTCGCAGCGCCTGATTCCCACCGAAGACGGCCAGGGCCGCGTTGCCGCAGTGGAAGTGCTGCTGAACA
CGCCGCTCATCTCCGACCTGATCTTCAAGGGCGAAGTGGGTGAGATCAAAGAGATCATGAAGAAGAGCCGGAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAAGACGCGATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTTTCGGCCGGTACGGAGC
ATTTCGCGATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baumannii D1279779

44.828

84.392

0.378
  pilT Acinetobacter baumannii strain A118

44.828

84.392

0.378
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

43.161

87.037

0.376
  pilT Legionella pneumophila strain ERS1305867

43.161

87.037

0.376

Multiple sequence alignment