Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GMX02_RS15635 Genome accession   NZ_CP046378
Coordinates   3536397..3537509 (-) Length   370 a.a.
NCBI ID   WP_025887558.1    Uniprot ID   A0A379YLK5
Organism   Shewanella algae strain RQs-106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3531397..3542509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMX02_RS15610 (GMX02_15610) - 3531443..3532573 (-) 1131 WP_146508520.1 aminotransferase class V-fold PLP-dependent enzyme -
  GMX02_RS15615 (GMX02_15615) - 3532584..3533318 (-) 735 WP_156020028.1 tryptophan 2,3-dioxygenase family protein -
  GMX02_RS15620 (GMX02_15620) hemH 3533494..3534507 (-) 1014 WP_044733989.1 ferrochelatase -
  GMX02_RS15625 (GMX02_15625) - 3534542..3535072 (-) 531 WP_414482966.1 glutathione peroxidase -
  GMX02_RS15630 (GMX02_15630) - 3535163..3536272 (-) 1110 WP_062793317.1 hypothetical protein -
  GMX02_RS15635 (GMX02_15635) pilU 3536397..3537509 (-) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GMX02_RS15640 (GMX02_15640) pilT 3537519..3538556 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  GMX02_RS15645 (GMX02_15645) - 3538593..3539288 (+) 696 WP_045282576.1 YggS family pyridoxal phosphate-dependent enzyme -
  GMX02_RS15650 (GMX02_15650) proC 3539421..3540239 (+) 819 WP_156020030.1 pyrroline-5-carboxylate reductase -
  GMX02_RS15655 (GMX02_15655) - 3540263..3540814 (+) 552 WP_129978769.1 YggT family protein -
  GMX02_RS15660 (GMX02_15660) yggU 3540814..3541101 (+) 288 WP_144377528.1 DUF167 family protein YggU -
  GMX02_RS15665 (GMX02_15665) - 3541164..3541598 (+) 435 WP_025887551.1 DUF4426 domain-containing protein -
  GMX02_RS15670 (GMX02_15670) rdgB 3541847..3542449 (+) 603 WP_144379939.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3709

>NTDB_id=403647 GMX02_RS15635 WP_025887558.1 3536397..3537509(-) (pilU) [Shewanella algae strain RQs-106]
MEVRPFLKAMVDRKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKDTMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=403647 GMX02_RS15635 WP_025887558.1 3536397..3537509(-) (pilU) [Shewanella algae strain RQs-106]
ATGGAAGTCAGACCTTTTCTCAAAGCCATGGTGGATCGCAAGGCCTCGGATCTCTTTATTACCGCCGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGCTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAGGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGCATCGAAACCAAGATCCCTACTGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGATTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTAGGGGGAACCGGTA
CGGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCTAATGCCAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCACTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCACCAACAACTGCTATTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCAGCGGCCGGCGCGCCGCCATCGAAGTGCTTATCAACACCCCCAGGGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGACACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
TTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCCGACTCGCCAAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAACTCAGGTTTCATGGAAGGAGTTACCCTGGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379YLK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

59.375

95.135

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362

Multiple sequence alignment